Skip to main content
SpringerLink
Log in

Actions of strigolactone GR24 and DRM1 gene expression on Arabidopsis root architecture

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Strigolactones are mostly known for their influence on apical dominance, but new insights suggest that they may be involved in many other biological events including root development. DRM1 gene is ubiquitary expressed in plants but its role is not well known. In our experiments, the strigolactone analogue GR24 stimulated the expression of DRM1.1, DRM1.2, DRM1.4 splicing variants and inhibited root branching in 5-day-old Arabidopsis thaliana (L.) Heynh. seedlings. On the other hand, the expression of these splicing variants was lower in 10-day-old GR24-treated roots. DRM1.6 gene expression differently responded to GR24 than other DRM1 splicing variants, however, there was no clear relationship between DRM1.6 expression and root length. Our results suggest that strigolactones and the expression of DRM1 gene play interactive roles in root branching.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DMSO:

dimethyl sulfoxide

DRM1 :

Dormancy associated gene-1

IDP:

intrinsically disordered protein

LR:

lateral roots

PAR:

photosynthetically active radiation

TAIR:

The Arabidopsis Information Resource

UBQ :

ubiquitine

UBC :

ubiquitine C

References

  1. Umehara, M., Hanada, A., Yoshida, S., Akiyama, K., Arite, T., Takeda-Kamiya, N., Magome, H., Kamiya, Y., Shirasu, K., Yoneyama, K., Kyozuka, J., and Yamaguchi, S., Inhibition of shoot branching by new terpenoid plant hormones, Nature, 2008, vol. 455, pp. 195–200.

    Article  CAS  PubMed  Google Scholar 

  2. Gomez-Roldan, V., Fermas, S., Brewer, P.B., Puech-Pages, V., Dun, E.A., Pillot, J.P., Letisse, F., Matousova, R., Danoun, S., Portais, J.C., Bouwmeester, H., Becard, G., Beveridge, C.A., Rameau, C., and Rochange, S.F., Strigolactone inhibition of shoot branching, Nature, 2008, vol. 455, pp. 189–194.

    Article  CAS  PubMed  Google Scholar 

  3. Brewer, P.B., Koltai, H., and Beveridge, C.A., Diverse roles of strigolactones in plant development, Mol. Plant, 2013, vol. 6, pp. 18–28.

    Article  CAS  PubMed  Google Scholar 

  4. Koltai, H., Dor, E., Hershenhorn, J., Joel, D. M., Weininger, S., Lekalla, S., Shealtiel, H., Bhattacharya, C., Eliahu, E., Resnik, N., Barg, R., and Kapulnik, Y., Strigolactones’ effect on root growth and root-hair elongation may be mediated by auxin-efflux carriers, J. Plant Growth Regul., 2010, vol. 29, pp. 129–136.

    Article  CAS  Google Scholar 

  5. Koltai, H. and Kapulnik, Y., Unveiling signalling events in root responses to strigolactone, Mol. Plant, 2013, vol. 6, pp. 589–591.

    Article  CAS  PubMed  Google Scholar 

  6. Koltai, H., Strigolactones are regulators of root development, New Phytol., 2011, vol. 190, pp. 545–549.

    Article  CAS  PubMed  Google Scholar 

  7. Umehara, M., Hanada, A., Yoshida, S., Akiyama, K., Arite, T., Takeda-Kamiya, N., Magome, H., Kamiya, Y., Shirasu, K., Yoneyama, K., Kyozuka, J., and Yamaguchi, S., Inhibition of shoot branching by new terpenoid plant hormones, Nature, 2008, vol. 455, pp. 195–200.

    Article  CAS  PubMed  Google Scholar 

  8. Gomez-Roldan, V., Fermas, S., Brewer, P.B., Puech-Pages, V., Dun, E.A., Pillot, J.P., Letisse, F., Matousova, R., Danoun, S., Portais, J.C., Bouwmeester, H., Becard, G., Beveridge, C.A., Rameau, C., and Rochange, S.F., Strigolactone inhibition of shoot branching, Nature, 2008, vol. 455, pp. 189–194.

    Article  CAS  PubMed  Google Scholar 

  9. Blume, Y.B., Yu, A., and Yemets, A.I., Effects of phytohormones on the cytoskeleton of the plant cell, Russ. J. Plant Physiol., 2012, vol. 59, pp. 515–529.

    Article  CAS  Google Scholar 

  10. Stafstrom, J.P., Ripley, B.D., Devitt, M.L., and Drake, B., Cloning and expression of PsDRM1 and PsDRM2, Planta, 1998, vol. 205, pp. 547–552.

    Article  CAS  PubMed  Google Scholar 

  11. Tatematsu, K., Ward, S., Leyser, O., Kamiya, Y., and Nambara, E., Identification of cis-elements that regulate gene expression during initiation of axillary bud outgrowth in Arabidopsis, Plant Physiol., 2005, vol. 138, pp. 757–766.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Rae, G.M., Uversky, V.N., David, K., and Wood, M., DRM1 and DRM2 expression regulation: potential role of splice variants in response to stress and environmental factors in Arabidopsis, Mol. Genet. Genomics, 2014, vol. 289, pp. 317–332.

    Article  CAS  PubMed  Google Scholar 

  13. Mastrangelo, A.M., Marone, D., Laidò, G., de Leonardis, A.M., and de Vita, P., Alternative splicing: enhancing ability to cope with stress via transcriptome plasticity, Plant Sci., 2012, vols. 185–186, pp. 40–49.

    Article  PubMed  Google Scholar 

  14. Ner-Gaon, H., Halachmi, R., Savaldi-Goldstein, S., Rubin, E., Ophir, R., and Fluhr, R., Intron retention is a major phenomenon in alternative splicing in Arabidopsis, Plant J., 2004, vol. 39, pp. 877–885.

    Article  CAS  PubMed  Google Scholar 

  15. Wright, P.E. and Dyson, H.J., Intrinsically disordered proteins in cellular signalling and regulation, Mol. Cell Biol., 2015, vol. 16, pp. 18–29.

    CAS  Google Scholar 

  16. Park, S. and Han, K.H., An auxin-repressed gene (RpARP) from black locust (Robinia pseudoacacia) is posttranscriptionally regulated and negatively associated with shoot elongation, Tree Physiol., 2003, vol. 23, pp. 815–823.

    Article  CAS  PubMed  Google Scholar 

  17. Rasmussen, A., Heugebaert, T., Matthys, C., van Deun, R., Boyer, F.D., Goormachtig, S., Stevens, C., and Geelen, D., A fluorescent alternative to the synthetic strigolactone GR24, Mol. Plant, 2013, vol. 6, pp. 100–112.

    Article  CAS  PubMed  Google Scholar 

  18. Shinohara, N., Taylor, C., and Leyser, O., Strigolactone can promote or inhibit shoot branching by triggering rapid depletion of the auxin efflux protein PIN1 from the plasma membrane, PloS Biol., 2013, vol. 11, no. 1, p. e1001474.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Central European Institute of Technology, Brno, 61300, Czech Republic

    A. Tripepi

  2. Department of Agriculture, Food and Environment, University of Pisa, Pisa, 56124, Italy

    L. Guglielminetti

Authors
  1. A. Tripepi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Guglielminetti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Tripepi.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tripepi, A., Guglielminetti, L. Actions of strigolactone GR24 and DRM1 gene expression on Arabidopsis root architecture. Russ J Plant Physiol 64, 845–849 (2017). https://doi.org/10.1134/S1021443717060127

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1021443717060127

Keywords

Search

Navigation