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Auto-inducible expression system based on the SigB-dependent ohrB promoter in Bacillus subtilis

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Abstract

A reliable production of heterologous proteins is important in the field of industrial biotechnology. This can be achieved by applying auto-inducible gene expression systems. Development of a Bacillus subtilis expression plasmid harboring SigB-dependent ohrB promoter was reported. The expression system was subjected to high cell density cultivation to produce xylanase as a stable model protein. The recombinant strain was cultured in a synthetic medium containing glucose as the carbon source. The exponential fed-batch feeding strategy was applied to prevent substrate inhibition. A sharp increase of xylanase activity (about 6-fold) at the end of fermentation was observed as a result of sigma factor B (SigB) protein activation, supporting autoinducibility of the expression system. For the control strain a specific induction of the xylanase activity was not observed. The recombinant strain showed a 5-fold increase in xylanase activity in comparison with the control strain. In addition, the constructed system displayed the catabolite repression resistance ability. This SigB-dependent expression system can be considered as a biotechnological tool and an alternative to the high costing conventional inducers, e.g. isopropyl-β-galactopyranoside.

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Authors and Affiliations

  1. Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-114, Tehran, Iran

    R. Panahi, E. Vasheghani-Farahani & S. A. Shojaosadati

  2. National Institute for Genetic Engineering and Biotechnology, Tehran, Iran

    B. Bambai

Authors
  1. R. Panahi
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  2. E. Vasheghani-Farahani
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  3. S. A. Shojaosadati
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  4. B. Bambai
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Correspondence to E. Vasheghani-Farahani.

Additional information

Published in Russian in Molekulyarnaya Biologiya, 2014, Vol. 48, No. 6, pp. 970–976.

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Panahi, R., Vasheghani-Farahani, E., Shojaosadati, S.A. et al. Auto-inducible expression system based on the SigB-dependent ohrB promoter in Bacillus subtilis . Mol Biol 48, 852–857 (2014). https://doi.org/10.1134/S0026893314060132

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  • DOI: https://doi.org/10.1134/S0026893314060132

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