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Molecular Analysis of the Rhizosphere Microbial Communities from Gramineous Plants Grown on Contrasting Soils

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Abstract—

The composition of rhizosphere microbial communities was studied for different species and varieties of agricultural gramineous plants (rye and wheat) grown in the soils contrasting in a number of agrochemical parameters. The data on analysis of the rhizosphere microbial communities obtained by sequencing of the V4 variable region of the 16S rRNA gene and on the ion and mass spectral composition of soil extracts are presented. Alpha diversity indices of the rhizosphere and control communities were almost the same. Analysis of beta diversity revealed higher variability of the rhizosphere microbial communities for chernozem-grown plants. Rhizosphere communities of the plants grown on sod-podzol soil were more similar and formed separate clusters. The taxa reliably involved in formation of specific rye and wheat rhizosphere microbiomes were established. Both common rhizosphere taxa (Sphingobacteriia, Betaproteobacteria) and species- or variety-specific ones (Oxalobacteraceae, Sphingobacteriaceae) were determined. Ion concentrations in soil extracts varied significantly depending on the soil type, while the similarity of mass spectral profiles depended more on the plant species than on the soil type.

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Funding

This work was supported by the Russian Science Foundation (project no. 41-26-00049P, “Analysis of the Genetic and Evolutionary Potential of Soil Microbiome for Increasing Plant Productivity and Soil Fertility”) using the equipment of the Genome Technologies and Cell Biology Common Use Center, All-Russian Research Institute of Agricultural Microbiology. The text of the article was prepared using the funds of the RSF project no. 18-16-00073.

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Authors and Affiliations

  1. All-Russian Research Institute of Agricultural Microbiology, 196608, St.-Petersburg, Russia

    A. O. Zverev, E. V. Pershina, V. M. Shapkin, A. K. Kichko, A. A. Belimov & E. E. Andronov

  2. Vavilov All-Russian Institute of Plant Genetic Resources, 190000, St.-Petersburg, Russia

    O. P. Mitrofanova & V. D. Kobylyanskii

  3. All-Russian Institute of Plant Protection, 196608, St.-Petersburg, Russia

    O. S. Yuzikhin

  4. St.-Petersburg State University, 199034, St.-Petersburg, Russia

    E. E. Andronov

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  1. A. O. Zverev
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  2. E. V. Pershina
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  3. V. M. Shapkin
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  4. A. K. Kichko
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  5. O. P. Mitrofanova
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  6. V. D. Kobylyanskii
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  7. O. S. Yuzikhin
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  8. A. A. Belimov
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  9. E. E. Andronov
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Correspondence to A. O. Zverev.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Babchenko

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Zverev, A.O., Pershina, E.V., Shapkin, V.M. et al. Molecular Analysis of the Rhizosphere Microbial Communities from Gramineous Plants Grown on Contrasting Soils. Microbiology 89, 231–241 (2020). https://doi.org/10.1134/S002626172001018X

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  • DOI: https://doi.org/10.1134/S002626172001018X

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