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Induction of lipid flip-flop by colicin E1 — a hallmark of proteolipidic pore formation in liposome membranes

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Abstract

The addition of the channel-forming domain of colicin E1 to liposomes elicited the transmembrane diffusion (flip-flop) of lipids concomitant to the release of the fluorescent dye from liposomes. Good correlation was found between kinetic and concentration dependences of the two processes. Both the liposome leakage and the lipid flip-flop were stimulated upon alkalinization of the buffer solution after colicin binding at acidic pH. These results in combination with the analysis of the data on colicin binding to liposomes provide evidence in favor of the validity of the toroidal (proteolipidic) pore model as the mechanism of colicin channel formation.

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Abbreviations

BLM:

bilayer lipid membrane

Br-PC:

9,10-dibromopalmitoylphosphatidylcholine

CF:

5(6)-carboxyfluorescein

DPhPC:

diphytanoyl phosphatidylcholine

DPhPG:

diphytanoyl phosphatidylglycerol

P178:

channel-forming domain of colicin E1

PyPC:

1-lauroyl-2-(1-pyrenebutyroyl)-sn-glycero-3-phosphatidylcholine

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Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    A. A. Sobko, E. A. Kotova & Y. N. Antonenko

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871, Moscow, Russia

    S. I. Kovalchuk

Authors
  1. A. A. Sobko
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  2. S. I. Kovalchuk
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  3. E. A. Kotova
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  4. Y. N. Antonenko
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Correspondence to A. A. Sobko.

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Original Russian Text © A. A. Sobko, S. I. Kovalchuk, E. A. Kotova, Y. N. Antonenko, 2010, published in Biokhimiya, 2010, Vol. 75, No. 6, pp. 819–826.

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Sobko, A.A., Kovalchuk, S.I., Kotova, E.A. et al. Induction of lipid flip-flop by colicin E1 — a hallmark of proteolipidic pore formation in liposome membranes. Biochemistry Moscow 75, 728–733 (2010). https://doi.org/10.1134/S0006297910060076

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  • DOI: https://doi.org/10.1134/S0006297910060076

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