Skip to main content
SpringerLink
Log in

The potential of scanning microcalorimetry for studying thermotropic conformational transitions in biomacromolecules1

  • Published:
Polymer Science Series C Aims and scope Submit manuscript

Abstract

General operational principles of a scanning microcalorimeter, methods and approaches to obtaining thermodynamic and kinetic information on the studied transition, and the existence of correlations between the structural features of a macromolecule and the measured thermodynamic parameters of denaturation transitions are considered. The results of studying the domain organization for a number of proteins and transfer poly(ribonucleic acids), the kinetics of heat denaturation, and the nature of the transitional (activated) state of macromolecules are surveyed. The potential of high-pressure scanning microcalorimetry for measuring volume changes during conformational transitions of proteins and lipids is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. L. Biltonen and E. Freire, Crit. Rev. Biochem. 5, 85 (1978).

    Article  CAS  Google Scholar 

  2. E. Freire and R. L. Biltonen, Biopolymers 17, 463 (1978).

    Article  CAS  Google Scholar 

  3. V. V. Filimonov, S. A. Potekhin, S. V. Matveev, and P. L. Privalov, Mol. Biol. (Moscow) 26, 551 (1982).

    Google Scholar 

  4. P. L. Privalov, Adv. Protein Chem. 35, 1 (1982).

    Article  CAS  Google Scholar 

  5. P. L. Privalov and S. A. Potekhin, Method. Enzymol. 131, 4 (1986).

    Article  CAS  Google Scholar 

  6. S.-J. Kidokoro and A. Wada, Biopolymers 26, 213 (1987).

    Article  CAS  Google Scholar 

  7. P. L. Privalov, V. V. Plotnikov, and V. V. Filimonov, J. Chem. Thermodyn. 7, 41 (1975).

    Article  CAS  Google Scholar 

  8. P. L. Privalov, Pure Appl. Chem. 52, 479 (1980).

    Article  CAS  Google Scholar 

  9. A. A. Senin, S. A. Potekhin, E. I. Tiktopulo, and V. V. Filimonov, J. Therm. Anal. Calorim. 62, 153 (2000).

    Article  CAS  Google Scholar 

  10. S. A. Potekhin and P. L. Privalov, J. Mol. Biol. 159, 519 (1982).

    Article  CAS  Google Scholar 

  11. S. A. Potekhin, V. A. Trapkov, and P. L. Privalov, Biofizika 24, 46 (1979).

    CAS  Google Scholar 

  12. S. A. Potekhin and P. L. Privalov, Mol. Biol. (Moscow) 13, 666 (1979).

    CAS  Google Scholar 

  13. V. M. Tishchenko, V. P. Zav’yalov, G. A. Medgyesi, S. A. Potekhin, and P. L. Privalov, Eur. J. Biochem. 126, 517 (1982).

    Article  CAS  Google Scholar 

  14. P. L. Privalov and L. V. Medved’, J. Mol. Biol. 159, 665 (1982).

    Article  CAS  Google Scholar 

  15. L. Medved, S. Litvinovich, T. Ugarova, Y. Matsuka, and K. Ingham, Biochemistry 36, 4685 (1997).

    Article  CAS  Google Scholar 

  16. G. Tsurupa, L. Tsonev, and L. Medved, Biochemistry 41, 6449 (2002).

    Article  CAS  Google Scholar 

  17. L. V. Medved, D. A. Solovjov, and K. C. Ingham, Eur. J. Biochem. 239, 333 (1996).

    Article  CAS  Google Scholar 

  18. L. V. Tatunashvili, V. V. Filimonov, P. L. Privalov, M. L. Metsis, V. E. Koteliansky, K. C. Ingham, and L. V. Medved, J. Mol. Biol. 211, 161 (1990).

    Article  CAS  Google Scholar 

  19. T. N. Tsalkova and P. L. Privalov, J. Mol. Biol. 181, 533 (1985).

    Article  CAS  Google Scholar 

  20. V. V. Novokhatny, S. A. Kudinov, and P. L. Privalov, J. Mol. Biol. 179, 215 (1984).

    Article  CAS  Google Scholar 

  21. P. L. Privalov and V. V. Filimonov, J. Mol. Biol. 122, 447 (1978).

    Article  CAS  Google Scholar 

  22. S. A. Potekhin, Biofizika 38, 47 (1993).

    CAS  Google Scholar 

  23. S. A. Potekhin, Biofizika 38, 239 (1993).

    CAS  Google Scholar 

  24. S. A. Potekhin and E. L. Kovrigin, Biofizika 43, 223 (1998).

    CAS  Google Scholar 

  25. S. A. Potekhin and E. L. Kovrigin, Biophys. Chem. 73, 241 (1998).

    Article  CAS  Google Scholar 

  26. S. A. Potekhin, O. I. Loseva, E. I. Tiktopulo, and A. P. Dobritsa, Biochemistry 38, 4121 (1999).

    Article  CAS  Google Scholar 

  27. S. A. Potekhin, K. Ogasahara, and K. Yutani, J. Therm. Anal. Calorim. 62, 111 (2000).

    Article  CAS  Google Scholar 

  28. T. Melnik, T. Povarnitsyna, H. Solonenko, and B. Melnik, Thermochim. Acta 512, 71 (2011).

    Article  CAS  Google Scholar 

  29. A. I. Dragan, S. A. Potekhin, A. Sivolob, M. Lu, and P. L. Privalov, Biochemistry 43, 14891 (2004).

    Article  CAS  Google Scholar 

  30. S. A. Potekhin, A. A. Senin, N. N. Abdurakhmanov, and R. S. Khusainova, Biochim. Biophys. Acta 1778, 2588 (2008).

    Article  CAS  Google Scholar 

  31. S. A. Potekhin, A. A. Senin, N. N. Abdurakhmanov, and R. S. Khusainova, Biochim. Biophys. Acta 1808, 1806 (2011).

    Article  CAS  Google Scholar 

  32. S. A. Potekhin, A. A. Senin, N. N. Abdurakhmanov, and E. I. Tiktopulo, Biochim. Biophys. Acta 1794, 1151 (2009).

    Article  CAS  Google Scholar 

  33. H. Heerklotz, J. Phys., Condens. Matter 16, R441 (2004).

    Article  CAS  Google Scholar 

  34. L. N. Lin, J. F. Brandts, J. M. Brandts, and V. Plotnikov, Anal. Biochem. 302, 144 (2002).

    Article  CAS  Google Scholar 

  35. P. L. Privalov and N. N. Khechinashvili, J. Mol. Biol. 86, 665 (1974).

    Article  CAS  Google Scholar 

  36. P. L. Privalov, Adv. Protein Chem. 33, 167 (1979).

    Article  CAS  Google Scholar 

  37. A. V. Finkel’shtein and O. B. Ptitsin, Physics of Protein (KDU, Moscow, 2005), p. 296.

    Google Scholar 

  38. E. Freire, W. W. Van Osdol, O. L. Mayorga, and J. M. Sanchez-Ruiz, Annu. Rev. Biophys. Biophys. Chem. 19, 159 (1990).

    Article  CAS  Google Scholar 

  39. J. M. Sanchez-Ruiz, in Differential Scanning Calorimetry of Proteins: Subcellular Biochemistry, Ed. by B. B. Biswas and S. Roy (Plenum, New York, 1995), p. 133.

    Google Scholar 

  40. O. Lopez-Mayorga and E. Freire, Biophys. Chem. 87, 87 (1987).

    Article  Google Scholar 

  41. Biophys. Chem. 51, 291 (1994).

  42. P. L. Privalov and G. I. Makhatadze, J. Mol. Biol. 213, 385 (1990).

    Article  CAS  Google Scholar 

  43. G. I. Makhatadze and P. L. Privalov, Adv. Protein Chem. 47, 307 (1995).

    Article  CAS  Google Scholar 

  44. J. M. Sanchez-Ruiz, Biophys. Chem. 148, 1 (2010).

    Article  CAS  Google Scholar 

  45. J. M. Sanchez-Ruiz, Biophys. J. 61, 921 (1992).

    Article  CAS  Google Scholar 

  46. D. Milardi, C. La Rosa, and D. Grasso, Biophys. Chem. 52, 183 (1994).

    Article  CAS  Google Scholar 

  47. A. E. Lyubarev and B. I. Kurganov, Recent Res. Dev. Biophys. Chem. 2, 141 (2001).

    CAS  Google Scholar 

  48. S. E. Zale and A. M. Klibanov, Biochemistry 25, 5432 (1986).

    Article  CAS  Google Scholar 

  49. D. I. Kreimer, V. L. Shnyrov, E. Villar, I. Silman, and L. Weiner, Protein Sci. 4, 2349 (1995).

    Article  CAS  Google Scholar 

  50. H. Heerklotz and J. Seelig, Biophys. J. 82, 1445 (2002).

    Article  CAS  Google Scholar 

  51. H. Ebel, P. Grabitz, and T. Heimburg, J. Phys. Chem. B 105, 7353 (2001).

    Article  CAS  Google Scholar 

  52. H. Heerklotz, Biophys. J. 83, 2693 (2002).

    Article  CAS  Google Scholar 

  53. P. Kujawa and F. M. Winnik, Macromolecules 34, 4130 (2001).

    Article  CAS  Google Scholar 

  54. A. Cooper, C. M. Johnson, J. H. Lakey, and M. Nollmann, Biophys. Chem. 93, 215 (2001).

    Article  CAS  Google Scholar 

  55. P. Grabitz, V. P. Ivanova, and T. Heimburg, Biophys. J. 82, 299 (2002).

    Article  CAS  Google Scholar 

  56. S. L. Wang and R. M. Epand, Chem. Phys. Lipids 129, 21 (2004).

    Article  CAS  Google Scholar 

  57. S. L. Randzio, Thermochim. Acta 398, 75 (2003).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Protein Research, Russian Academy of Sciences, Institutskaya ul. 4, Pushchino, Moscow oblast, 142290, Russia

    S. A. Potekhin

Authors
  1. S. A. Potekhin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. A. Potekhin.

Additional information

Original Russian Text © S.A. Potekhin, 2012, published in Vysokomolekulyarnye Soedineniya, Ser. C, 2012, Vol. 54, No. 7, pp. 1077–1085.

This work was supported by the Russian Foundation for Basic Research, project no. 11-04-00864-a.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Potekhin, S.A. The potential of scanning microcalorimetry for studying thermotropic conformational transitions in biomacromolecules1. Polym. Sci. Ser. C 54, 108–115 (2012). https://doi.org/10.1134/S1811238212070053

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1811238212070053

Keywords

Search

Navigation