Skip to main content
SpringerLink
Log in

Solution of the problem of nonspecific binding in solid-phase noninstrumental dot immunoassay

  • Biochemistry, Biophysics and Molecular Biology
  • Published:
Doklady Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

In this work, we evaluated blocking properties of various reagents used in dot immunoassay test systems on nitrocellulose membranes employing carbon-based diagnosticum to determine antibodies to tetanus anatoxin, thermostable toxin of Yersinia pseudotuberculosis, and HIV-I. The results support the usage of a nonionic detergent Tween 20 which efficiently inhibited nonspecific protein binding to the solid-phase reagent surface and did not decrease sensitivity of the analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Plaksin, D.Yu., Raev, M.B., and Gromakovskaya, E.T., RF Patent 20 899 212, Byull. Izobret., 1997, no. 25.

    Google Scholar 

  2. Esser, P., Thermo Sci. Nunc. Bull., 2010, no. 9, pp. 1–4.

    Google Scholar 

  3. Esser, P., Thermo Sci. Nunc. Bull., 1990, no. 8, pp. 1–5.

    Google Scholar 

  4. Huber, D., Rudolf, J., Ansari, P., et al., Anal. Bioanal. Chem., 2009, vol. 394, no. 2, pp. 539–548.

    Article  PubMed  CAS  Google Scholar 

  5. Morissette, C., Goulet, J., and Lamoureux, G., Appl. Environ. Microbiol., 1991, vol. 57, no. 3, pp. 836–842.

    PubMed  CAS  Google Scholar 

  6. Rayev, M. and Shmagel, K., J. Immunol. Methods, 2008, vol. 336, no. 1, pp. 9–15.

    Article  PubMed  CAS  Google Scholar 

  7. Vogt, R.F., Phillips, D.L., Henderson, L.O., et al., J. Immunol. Methods, 1987, vol. 101, no. 1, pp. 43–50.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, Russia

    M. S. Bochkova, V. P. Timganova & M. B. Raev

Authors
  1. M. S. Bochkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. P. Timganova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. B. Raev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. S. Bochkova.

Additional information

Original Russian Text © M.S. Bochkova, V.P. Timganova, M.B. Raev, 2013, published in Doklady Akademii Nauk, 2013, Vol. 449, No. 1, pp. 101–103.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bochkova, M.S., Timganova, V.P. & Raev, M.B. Solution of the problem of nonspecific binding in solid-phase noninstrumental dot immunoassay. Dokl Biochem Biophys 449, 63–65 (2013). https://doi.org/10.1134/S1607672913020014

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1607672913020014

Keywords

Search

Navigation