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Development of a novel electrochemical biosensor based on catalytic properties of adenosine deaminase immobilized on graphene oxide/carboxymethyl chitosan/multi-wall carbon nanotube platform

  • Technology of Electrochemical Industry
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Abstract

A new type of biosensor was designed based on Adenosine deaminase (ADA) immobilized on graphene oxide (GO)/carboxymethyl chitosan (CMC)/multi-wall carbon nanotube (MWCNT) platform nanostructure, fabricated and successfully applied (utilized) in Adenosine detection. Square wave voltammetry was used to study the biosensor catalytic activity. Morphological analysis of the nanostructure was performed by AFM and SEM methods. The results provided here proved that utilizing GO/CMC/MWCNT leads to effective immobilization of ADA which was confirmed by the long term stability of the biosensor during examined intervals. The immobilized ADA activity was examined and the kinetic parameters (K m and V max) were found to be 47.5 μM and 5.8 μM min−1, respectively. Furthermore, benznidazole was introduced as a potent ADA inhibitor using virtual screening. Outstanding inhibition characteristics of benznidazole was observed against ADA. ADA inhibition by benznidazole was non-competitive with the inhibition constant of 0.42 μM. Such an interesting template with an easy preparation process with low cost can provide a novel matrix for developing biosensors and biocatalysts based on enzyme immobilization.

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Authors and Affiliations

  1. Chemical Engineering Department, Monash University, Clayton Campus, Melbourne, Australia

    S. Taranejoo

  2. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 1985717443, Iran

    S. Taranejoo

  3. Kashan Branch, Islamic Azad University, Kashan, Iran

    S. Taranejoo & M. Moghri

Authors
  1. S. Taranejoo
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  2. M. Moghri
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Correspondence to S. Taranejoo.

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Original English Text © S. Taranejoo, M. Moghri, 2014, published in Zhurnal Prikladnoi Khimii, 2014, Vol. 87, No. 1, pp. 74–80.

The text was submitted by author in English.

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Taranejoo, S., Moghri, M. Development of a novel electrochemical biosensor based on catalytic properties of adenosine deaminase immobilized on graphene oxide/carboxymethyl chitosan/multi-wall carbon nanotube platform. Russ J Appl Chem 87, 69–75 (2014). https://doi.org/10.1134/S1070427214010108

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  • DOI: https://doi.org/10.1134/S1070427214010108

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