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Bacteriophage enzymes for the prevention and treatment of bacterial infections: Stability and stabilization of the enzyme lysing Streptococcus pyogenes cells

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Abstract

The effect of various compounds on the activity and stability of a phage-associated enzyme lysing cells of streptococci of groups A and C (PlyC) was investigated. Substantial inhibition of the enzyme activity was revealed at an increased ionic strength (in the presence of NaCl) and upon the addition of carbohydrates (mono-, di-, and polysaccharides), i.e., agents stabilizing many enzymes. It was established that the enzyme activity was substantially reduced in the presence of positively charged polyelectrolytes and surfactants, whereas incubation with micelle-forming substances and negatively charged polyelectrolytes led to PlyC activation and stabilization. It was shown that, in the micellar polyelectrolyte composition M16, the enzyme retained its activity for 2 months; while in a buffer solution under the same conditions (pH 6.3, room temperature), ture), it practically completely lost its activity in 2 days. Characteristics of the enzyme thermal inactivation were found, in particular, its half-inactivation time at various temperatures; these allowed us to estimate its behavior at any temperature and to recommend conditions for its storage and use.

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Abbreviations

CPC:

cetylpyridinium chloride

PAA:

polyacrylic acid

PlyC:

phage-associated enzyme lysing streptococcal cells of A and C groups

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Authors and Affiliations

  1. Faculty of Chemistry, Moscow State University, Vorobyevy gory, Moscow, 119992, Russia

    N. L. Klyachko, A. S. Eshchina, O. V. Ignatenko, L. Yu. Filatova, E. I. Rainina, A. K. Kazarov & A. V. Levashov

  2. Faculty of Preventive Medicine, Sechenov Moscow Medical Academy, Trubetskaya ul. 8-2, Moscow, 119992, Russia

    N. F. Dmitrieva

  3. Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA, 99352, USA

    E. I. Rainina

Authors
  1. N. L. Klyachko
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  2. N. F. Dmitrieva
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  3. A. S. Eshchina
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  4. O. V. Ignatenko
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  5. L. Yu. Filatova
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  6. E. I. Rainina
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  7. A. K. Kazarov
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  8. A. V. Levashov
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Corresponding author

Correspondence to N. L. Klyachko.

Additional information

Original Russian Text © N.L. Klyachko, N.F. Dmitrieva, A.S. Eshchina, O.V. Ignatenko, L.Yu. Filatova, E.I. Rainina, A.K. Kazarov, A.V. Levashov, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 3, pp. 416–421.

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Klyachko, N.L., Dmitrieva, N.F., Eshchina, A.S. et al. Bacteriophage enzymes for the prevention and treatment of bacterial infections: Stability and stabilization of the enzyme lysing Streptococcus pyogenes cells. Russ J Bioorg Chem 34, 375–379 (2008). https://doi.org/10.1134/S1068162008030217

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  • DOI: https://doi.org/10.1134/S1068162008030217

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