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Role of sensitivity of zinc oxide nanorods (ZnO-NRs) based photosensitizers in hepatocellular site of biological tissue

  • Laser Methods In Chemistry, Biology, and Medicine
  • Published:
Laser Physics

An Erratum to this article was published on 02 September 2011

Abstract

Zinc oxide nanorods (ZnO-NRs) with high surface to volume ratio and bio compatibility are used as an efficient photosensitizer carrier system for achievement of Hepatocellular cancer cell (HepG2) necrosis within few minutes. Present study highlights the role of effectiveness of ZnO-NRs in photodynamic therapy (PDT). We have grown the ZnO-NRs on the tip of borosilicate glass capillaries (0.5 μm diameter). The grown ZnO-NRs were conjugated using Photofrin® and ALA for the efficient intracellular drug delivery, which produces reactive oxygen species (ROS) via photochemical reactions leading to cell death within few minutes after exposing UV light (240 nm). Viability of controlled and treated HepG2 cells with optimum dose of light (UV-visible) has been assessed by neutral red assay (NRA). The results were verified by staining of mitochondria using Mitotracker® red as an efficient dye as well as ROS detection. ZnO-NRs based Phogem® (PG) treated normal liver tissues of Sprague-Dawley rats were used as comparative experimental model. Morphological apoptotic changes in liver tissue of Sprague-Dawley rats before and after ZnO-NRs conjugated with photosensitizer (PS)-mediated PDT were investigated by microscopic examination.

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Authors and Affiliations

  1. Research Chair for Laser Diagnosis of Cancer, Physics and Astronomy Department, College of Science, King Saud University, Riyadh, Saudi Arabia

    M. Atif & M. S. AlSalhi

  2. Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, 45650, Pakistan

    M. Atif & M. Fakhar-e-Alam

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  1. M. Atif
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  2. M. Fakhar-e-Alam
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Original Text © Astro, Ltd.

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An erratum to this article can be found at http://dx.doi.org/10.1134/S1054660X12040354.

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Atif, M., Fakhar-e-Alam, M. & AlSalhi, M.S. Role of sensitivity of zinc oxide nanorods (ZnO-NRs) based photosensitizers in hepatocellular site of biological tissue. Laser Phys. 21, 1950–1961 (2011). https://doi.org/10.1134/S1054660X11190029

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