Abstract
Avian reovirus (ARV) structural protein, σC, the prime candidate for vaccine against ARV, was expressed using a baculovirus/insect cell system. The expressed protein remained intracellular and reached 96 μg/106 cells. Total product yield from a 200 ml suspension culture was 19 mg. When the protein was fused with a histidine tag and an enterokinase (EK) cleavage site, purification of 94% was achieved in a single step. The histidine tag was removed by EK.
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Hu, YC., Liu, HJ. & Chung, YC. High level expression of the key antigenic protein, σC, from avian reovirus into insect cells and its purification by immobilized metal affinity chromatography. Biotechnology Letters 24, 1017–1022 (2002). https://doi.org/10.1023/A:1015689313560
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DOI: https://doi.org/10.1023/A:1015689313560