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Equilibrium protein folding-unfolding process involving multiple intermediates

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Abstract

Mathematical models for the protein folding-unfolding process involving multiple intermediates have been derived. Computer fitting of the experimental data to this model generates various thermodynamic parameters for the folding-unfolding process. In this way, the complex folding-unfolding process of the multi-domain proteins can be analysed in a quantitative way. The application of the folding-unfolding model involving seven stages in human placental alkaline phosphatase is described.

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Authors and Affiliations

  1. Department of Life Science, National Chung-Hsing University, Taichung, Taiwan

    Hui-Chih Hung

  2. Institutes of Life Science and Biochemistry, National Defense Medical Center, Taipei, Taiwan

    Yu-Hou Chen & Hwei-Jen Lee

  3. Institute of Immunology, Chung-Shan Medical University, Taichung, Taiwan

    Guang-Yaw Liu

  4. Department of Life Science, National Yang-Ming University, Shih-Pai, 112, Taipei, Taiwan

    Gu-Gang Chang

Authors
  1. Hui-Chih Hung
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  2. Yu-Hou Chen
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  3. Guang-Yaw Liu
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  4. Hwei-Jen Lee
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  5. Gu-Gang Chang
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Correspondence to Gu-Gang Chang.

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These authors contributed equally to this paper.

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Hung, HC., Chen, YH., Liu, GY. et al. Equilibrium protein folding-unfolding process involving multiple intermediates. Bull. Math. Biol. 65, 553–570 (2003). https://doi.org/10.1016/S0092-8240(03)00024-7

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  • DOI: https://doi.org/10.1016/S0092-8240(03)00024-7

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