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Effect of Collagen-Polycaprolactone Nanofibers Matrix Coating on the In Vitro Cytocompatibility and In Vivo Bone Responses of Titanium

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Abstract

The objective of this study was to improve the biomechanical performance of titanium (Ti) using a biocompatible electrospun nanofiber matrix. The study is based on the hypothesis that coating a Ti surface with a nanofiber matrix (NFM) made of collagen (CG) and polycaprolactone (PCL) electrospun nanofibers could increase the mechanical fixation of Ti/bone by improving the surface and cytocompatibility properties of Ti. This study prepared Ti samples with and without CG-PCL NFM coatings. This study determined the in vitro effects of each group of Ti samples on the surface topography and cytocompatibility (osteoblast cell adhesion, proliferation, mineralization and protein adsorption) properties. This study also determined in vivo interface shear strength and bone volume fraction of each group of Ti samples with bone using a rabbit model. This study found that the CG-PCL NFM coating on Ti improved the surface roughness, osteoblast cell adhesion, proliferation, mineralization and protein adsorption properties of Ti. In vivo studies found that interface shear strength of CG-PCL NFM-coated Ti/bone samples was significantly higher compared to those values of control Ti/bone samples (p value < 0.05) due to an increase in the amount of growth of the connective tissue joining the Ti implant. Therefore, the developed CG-PCL NFM coating technique should further be investigated for its potential in clinical applications.

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Acknowledgements

This publication was made possible by Grant Number 5P20GM103447 from the NIH and on-campus faculty Grant and CURE-STEM awards from the College of Math and Science and the University of Central Oklahoma Office of Research and Grants. The AON-1 osteonectin antibody developed by John D. Termine was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. Special acknowledgment to Andria Hedrick, Research Imaging Facility at the College of Pharmacy in the University of Oklahoma Health Science Center, for µCT analysis support.

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Authors and Affiliations

  1. Department of Engineering & Physics, University of Central Oklahoma, Edmond, OK, 73034, USA

    Morshed Khandaker & Shahram Riahinezhad

  2. Department of Mathematics & Statistics, University of Central Oklahoma, Edmond, OK, 73034, USA

    Fariha Sultana & Tracy Morris

  3. Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA

    Roman Wolf

  4. Department of Biology, University of Central Oklahoma, Edmond, OK, 73034, USA

    Melville Vaughan

  5. Center for Interdisciplinary Biomedical Education and Research, University of Central Oklahoma, Edmond, OK, 73034, USA

    Morshed Khandaker, Shahram Riahinezhad, Fariha Sultana, Tracy Morris & Melville Vaughan

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  1. Morshed Khandaker
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Correspondence to Morshed Khandaker.

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Khandaker, M., Riahinezhad, S., Sultana, F. et al. Effect of Collagen-Polycaprolactone Nanofibers Matrix Coating on the In Vitro Cytocompatibility and In Vivo Bone Responses of Titanium. J. Med. Biol. Eng. 38, 197–210 (2018). https://doi.org/10.1007/s40846-017-0312-7

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  • DOI: https://doi.org/10.1007/s40846-017-0312-7

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