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Swimming training attenuates pancreatic apoptosis through miR-34a/Sirtu in1/P53 Axis in high-fat diet and Streptozotocin-induced Type-2 diabetic rats

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Abstract

Objective

The present study sought to evaluate the miR-34a/Sirtuin1/p53 pro-apoptotic pathway, and reveal its modulation in diabetic rats undergoing swimming exercise.

Methods

Twenty-eight male Wistar rats were divided into four groups. They were inducted to develop diabetes by injection of streptozotocin. After 12 weeks of swimming, the pancreatic tissue of these rats were removed to be evaluated for the expression level of Sitruin1/P53/miR-34a through qPCR.

Results

Findings indicated a marked rise in the expression of miR-34 and P53 (P < 0.01) as well as a significant decrease in expression of Sitruin1 (P < 0.01) in the diabetic group. In contrast, swimming resulted in a significant decrease in miR-34a expression (P < 0.01), and a prominent rise in the level of Sitruin1 in the swimming-trained-diabetic group (P < 0.01). Additionally, high, moderate and low apoptosis rate were observed in the pancreatic tissue of the diabetic, swimming-trained diabetic, and control groups, respectively.

Conclusion

Our findings suggested a correlation between pancreatic tissue apoptosis rate and miR-34a/Sitruin1/p53 signaling, that was subject to modulation by training.

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Acknowledgments

This study was endorsed by Liver and Gastrointestinal Diseases Research Center of Tabriz University of Medical Sciences (project No: 5/4/610). Special thanks for Milad Shirvaliloo editing job on the manuscript.

Funding

This study was funded by Liver and Gastrointestinal Diseases Research Center of Tabriz University of Medical Sciences (project No: 5/4/610).

Author information

Authors and Affiliations

  1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Reza Alipour & Rafighe Ghiasi

  2. Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Reza Alipour & Rafighe Ghiasi

  3. Nephrology and Kidney Transplant Research Center, Urmia University of Medical Science, Urmia, Iran

    Roya Naderi

  4. Department of Physiology, Urmia Faculty of Medical Science, Urmia, Iran

    Roya Naderi

  5. Department of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Alireza Alihemmati

  6. Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

    Roghayeh Sheervalilou

Authors
  1. Mohammad Reza Alipour
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  2. Roya Naderi
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  3. Alireza Alihemmati
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  4. Roghayeh Sheervalilou
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  5. Rafighe Ghiasi
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Contributions

Mohammad Reza Alipour and Rafighe Ghiasi designed the project. Rafighe Ghiasi, Roya Naderi and Alireza Alihemmati performed the required tests. Rafighe Ghiasi and Roghayeh Sheervalilou performed statistical analysis, and prepared the manuscript draft and revised it.

Corresponding author

Correspondence to Rafighe Ghiasi.

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The authors have affirmed that there is no conflict of interest.

Ethical issues

The protocol for this study was planned to follow the guidelines of NIH, and be in agreement with Ethics Committee for the Use of Animals in Research at Tabriz University of Medical Sciences (project No: 5/4/610).

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Alipour, M.R., Naderi, R., Alihemmati, A. et al. Swimming training attenuates pancreatic apoptosis through miR-34a/Sirtu in1/P53 Axis in high-fat diet and Streptozotocin-induced Type-2 diabetic rats. J Diabetes Metab Disord 19, 1439–1446 (2020). https://doi.org/10.1007/s40200-020-00670-6

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  • DOI: https://doi.org/10.1007/s40200-020-00670-6

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