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Alleviation of the hepatotoxic effect of bromobenzene by the Indian traditional herbal formulation Triphala in experimental rats

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Abstract

The present study is an attempt to investigate the efficacy of Triphala in ameliorating the hepatotoxic effects of bromobenzene (BB) in experimental rats. Hepatotoxicity was induced in rats by oral administration of BB (1.57 mg/kg b.w.) and Triphala was given orally at two doses of 250 and 500 mg/kg b.w. for a period of 8 days. The antioxidant status was studied by measuring lipid peroxidation, total reduced glutathione levels and enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) in liver tissue homogenates. In addition, the levels of cytokines such as tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were measured in serum. The hepatoprotective role of Triphala was compared with that of the standard hepatoprotective agent silymarin. The treatment of BB-intoxicated rats with Triphala showed significant (p < 0.05) increase in the antioxidant status and significant (p < 0.05) reduction in the levels of Tumour Necrosis Factor-α (TNF-α), Interleukin-1β (IL-1β). The histopathological findings further confirmed the protective role of Triphala against BB-induced hepatotoxicity in rats. From the results obtained in this study, it can be concluded that treatment with Triphala alleviated the hepatotoxic effect of BB in experimental rats.

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Acknowledgments

The authors are thankful to VIT University for lab facilities and infrastructure.

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The authors do not have any conflict of interest.

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Authors and Affiliations

  1. School of Biosciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India

    Udhaya Lavinya Baskaran, Mahaboobkhan Rasool & Evan Prince Sabina

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  1. Udhaya Lavinya Baskaran
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  2. Mahaboobkhan Rasool
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  3. Evan Prince Sabina
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Correspondence to Evan Prince Sabina.

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Baskaran, U., Rasool, M. & Sabina, E.P. Alleviation of the hepatotoxic effect of bromobenzene by the Indian traditional herbal formulation Triphala in experimental rats. Orient Pharm Exp Med 14, 369–374 (2014). https://doi.org/10.1007/s13596-014-0170-6

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  • DOI: https://doi.org/10.1007/s13596-014-0170-6

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