Abstract
Picrorhiza kurroa Royle ex Benth (Family: Scrophulariaceae) is a medicinal herb, mainly found in the North-Western Himalayas. Extensive harvesting for pharmaceutical purposes, lack of organized cultivation and unorganized methods of uprooting the plants because of unawareness has brought an endangered status to this important herb in nature. The medicinal property of this plant is attributed to monoterpenoid picrosides. The influence of developmental status of different growth stages on picrosides content is poorly understood in Picrorhiza kurroa. Picroside-I (P-I) content increased from 0.05 % to 0.76 % in different growth stages of shoots. Significant increase in the contents of P-I (0.15–0.50 %) and Picroside-II (P-II) (0.1–0.45 %) was observed in rhizomes of different developmental stages. Highest amounts of P-I (8.7 %) and P-II (5.3 %) was detected in uppermost part of mature dried rhizomes compared to bottom part with 2.9 % and 2.2 % of P-I and P-II, respectively. P. kurroa grown at high altitude (Sairopa, 4,500 amsl) showed 1.75-folds increase in P-I in leaves whereas exponential increase in the P-I content was detected (0.05–1.7 %) in the leaves of different developmental stages (L1-L5) of P. kurroa grown at lower altitude (Jagatsukh, 1,900 m). Variable amounts of P-I and P-II in different growth and developmental stages of P. kurroa imply importance of selection of plant material (rhizomes and roots). The study undertaken explored the status of metabolites accumulation and biosynthesis in the field grown plants of P. kurroa where not only environmental parameters but different morphogenetic stages of its developmental cycles, different age groups and different parts of plantlets were extensively analysed and estimated for medicinally important picrosides.
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Abbreviations
- P-I:
-
Picroside-I
- P-II:
-
Picroside-II
- HPLC:
-
High performance liquid chromatography
References
Ansari RA, Tripathi SC, Patnaik GK, Dhawan BN (1991) Antihepatotoxic properties of Picroliv, an active fraction from the rhizome of P. kurroa. J Ethnopharmacol 34(61)
Ansari RA, Aswal BS, Chander R (1998) Hepatoprotective activity of kutkin-the iridoid glycoside mixture of Picrorhiza kurroa. Indian J Med Res 87:401–404
Bhandari P, Kumar N, Singh B, Gupta AP, Kaul VK, Ahuja PS (2008) Stability Indicating LC-PDA method for determination of Picrosides in hepatoprotective Indian herbal preparations of Picrorhiza kurroa. Chromatographia 69:221–227
Chander R, Kapoor NK, Dhawan BN (1992) Picroliv, P-I and Kutkoside from P. kurroa are scavengers of superoxide anions. Biochem Pharmacol 44:180–183
Chettri N, Sharma E, Lama SD (2005) Non-timber forest produces utilization, distribution and status in a trekking corridor of Sikkim. Lyonia 8:89–101, India
Cromwell BT (1933) Experiments on the origin and function of Berberine in Berberis darwinii. Biochem J 27:860–872
Dorch W, Stuppner H, Wagner H (1991) Anti-asthamatic effects of P. kurroa: Androsin prevents allergen and PAF induced bronchial obstruction in guinea pig. Int Arch Allergy Appl Immunol 95:128–133
Dutt S, Kiddle G, Singh B, Khambay B, Foyer CH (2004) Differential accumulation of Picrosides in P. kurroa Royle ex Benth plants. www.rothamsted-international.org/files/posters/Posters/Sondutt/pdf
Dwivedi Y, Rastogi R, Garg NK, Dhawan BN (1992) Picroliv and its components Kutkoside and P I protect liver against galactosamine-induced damage in rats. Pharmacol Toxicol 71:383–387
Dwivedi AK, Kulkarni D, Singh S (1997) Sensitive high-performance liquid chromatographic assay method for the determination of P-I in plasma. J Chromatogr B Biomed Sci Appl 698:317–320
Gairola S, Shariff NM, Bhatt A, Kala CP (2010) Influence of climate change on production of secondary chemicals in high altitude medicinal plants: issues needs immediate attention. J Med Plant Res 4:1825–1829
Gupta A, Khajuria A, Singh J, Suri KA, Qazi GN (2006) Immunomodulatory activity of bipolymeric fraction RLJ-NE-205 from Picrorhiza kurroa. Int Immunopharmacol 6:1543–1549
Gupta N, Sharma SK, Rana JC, Chauhan RS (2011) Expression of flavonoid biosynthesis genes vis-à-vis rutin content variation in different growth stages of Fagopyrum species. J Plant Physiol 168:2117–2123
Joy KL, Rajeshkumar NV, Kuttan R (2000) Effect of Picrorhiza kurroa extract on transplanted tumours and chemical carcinogenesis in mice. J Ethnopharmacol 71:261–266
Katoch M, Fazli IS, Suri KA, Ahuja A, Qazi GN (2011) Effect of altitude on P content in core collections of Picrorhiza kurroa from the north western Himalayas. J Nat Med 65:578–582
Kowalski R, Wolski T (2006) Evaluation of phenolic acid content in Silphium perfoliatum L., leaves, inflorescences and rhizomes. Electron J Pol Agric Univ 6(1)
Kumar VH, Shashidhara NS, Rajendra CE (2010) Resperpine content of Rauwolfia serpentina in response to geographical variation. Int J Pharm Biol Sci 1:429
Nautiyal MC (1986) Physiology of reproduction in two Aconitum species. Ph.D. Thesis, H.N.B. Garhwal University, Srinagar
Nayar MP, Sastri ARK (1990) Red Data Plants of India. CSIR Publication, New Delhi, p 271
Nikolic GS, Zlatkovic SZ (2010) Assaying the variation in secondary metabolites of St. John’s wort for its better use as an antibiotic. J Med Plant Res 4:211–224
Palama TL, Khatib A, Choi YH, Payet B, Fock I, Verpoorte R, Kodja H (2009) Metabolic changes in different developmental stages of Vanilla planifolia pods. J Agric Food Chem 57:7651–7658
Pandey H, Nandi SK, Kumar A, Palni UT, Palni LMS (2007) Podophyllotoxin content in Podophyllum hexandrum Royle plants of known age of seed origin and grown at a lower altitude. Acta Physiol Plant 29:121–126
Praveen N, Naik PM, Manohar SH, Murthy HN (2010) Distribution of withanolide A content in various organs of Withania somnifera (L.) Dunal. Int J Pharm Biol Sci 1:1–5
Puri A, Saxena RP, Sumati, Guru PY, Kulshreshtha DK, Saxena KC, Dhawan BN (1992) Immunostimulant activity of Picroliv, the iridoid glycoside fraction of Picrorhiza kurroa, and its protective action against Leishmania donovani infection in hamsters. Planta Med 58:528–532
Rai LK, Prasad P, Sharma E (2000) Conservation threats to some important medicinal plants of the Sikkim Himalaya. Biol Conserv 93:27–53
Raina R, Mehra TS, Chand R, Sharma YP (2010) Reproductive biology of Picrorhiza kurroa a critically endangered high value temperate medicinal plant. J Med Aromat Plants 1:40–43
Singh V, Banyal HS (2011) Antimalarial effects of Picrorhiza kurroa Royle Ex Benth Extracts on Plasmodium berghei. Asian J Exp Biol Sci 2:529–532
Sood H, Chauhan RS (2010) Biosynthesis and accumulation of a medicinal compound, P-I, in cultures of Picrorhiza kurroa Royle ex Benth. Plant Cell Tissue Organ Cult 100:113–117
Sood H, Chauhan RS (2011) Different tissue culture parameters used for increased shoot biomass and its enrichment for a medicinal compound, P-1 in an endangered herb, Picrorhiza kurroa Royle ex Benth. Res Bioscientia 2:13–22
Stuppner H, Wagner H (1989) New cucurbitacin glycosides from Picrorhiza kurroa. Planta Med 55:559–560
Verma P, Basu V, Gupta V, Saxena G, Rahman L (2009) Pharmacology and chemistry of a potent hepatoprotective compound Picroliv isolated from the roots and rhizomes of Picrorrhiza kurroa Royle ex Benth (Kutki). Curr Pharm Biotechnol 10:641–649
Weinges K, Kloss P, Henkles WD (1972) Natural products from medicinal plants. XVII. P-II, a new 6-vanilloyl-catpol from Picrorhiza kurroa Royle and Benth. Justus Liebigs Ann Chem 759:173–182
Acknowledgements
The authors are thankful to the National Medicinal Plants Board, Ministry of Health, Govt. of India and the Jaypee University of Information Technology for providing financial assistance. We are also thankful to Dr. Jagdish Singh, Scientist, Himalayan Forest Research Institute, Panthaghati, Shimla, India for providing plant material of P. kurroa.
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Pandit, S., Shitiz, K., Sood, H. et al. Differential biosynthesis and accumulation of picrosides in an endangered medicinal herb Picrorhiza kurroa . J. Plant Biochem. Biotechnol. 22, 335–342 (2013). https://doi.org/10.1007/s13562-012-0136-z
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DOI: https://doi.org/10.1007/s13562-012-0136-z