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Physiological and proteomic analysis of plant growth enhancement by the rhizobacteria Bacillus sp. JS

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Abstract

In this study, the effects of the plant growth-promoting rhizobacterium (PGPR), Bacillus sp. JS on the growth of tobacco (Nicotiana tabacum ‘Xanthi’) and lettuce (Lactuca sativa ‘Crispa’), were evaluated by comparing various growth parameters between plants treated with the bacterium and those exposed to water or nutrient broth as control. In both tobacco and lettuce, fresh weight and length of shoots were increased upon exposure to Bacillus sp. JS. To explain the overall de novo expression of plant proteins by bacterial volatiles, two-dimensional gel electrophoresis was performed on samples from PGPR-treated tobacco plants. Our results showed that chlorophyll a/b binding proteins were significantly up-regulated, and total chlorophyll content was also increased. Our findings indicate the potential benefits of using Bacillus sp. JS as a growth-promoting factor in agricultural practice, and highlight the need for further research to explore these benefits.

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Acknowledgements

This work was supported by a grant (Code # S211214L030210) from Forest Science & Technology Projects, Forest Service and by Advanced Production Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (Code # 1100345), Republic of Korea.

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Authors and Affiliations

  1. Department of Environmental Horticulture, The University of Seoul, Seoulsiripdae-ro 163, Dongdaemun-gu, Seoul, 130-743, South Korea

    Ji Seong Kim, Jeong Eun Lee, Hualin Nie, Yong Jae Lee & Sun-Hyung Kim

  2. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan

    Ji Seong Kim & Jeong Eun Lee

  3. Department of Plant Bioscience, Pusan National University, Miryang, 627-706, South Korea

    Sun Tae Kim

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  1. Ji Seong Kim
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  5. Sun Tae Kim
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Correspondence to Sun-Hyung Kim.

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Ji Seong Kim declares that he has no conflict of interest. Jeong Eun Lee declares that he has no conflict of interest. Hualin Nie declares that he has no conflict of interest. Yong Jae Lee declares that he has no conflict of interest. Sun Tae Kim declares that he has no conflict of interest. Sun-Hyung Kim declares that he has no conflict of interest.

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Kim, J.S., Lee, J.E., Nie, H. et al. Physiological and proteomic analysis of plant growth enhancement by the rhizobacteria Bacillus sp. JS. Genes Genom 40, 129–136 (2018). https://doi.org/10.1007/s13258-017-0615-7

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  • DOI: https://doi.org/10.1007/s13258-017-0615-7

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