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Microwell-mediated micro cartilage-like tissue formation of adipose-derived stem cell

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Abstract

In cartilage tissue engineering, various technical approaches using postnatal stem cells, three-dimensional (3D) scaffolds composed of synthetic or natural polymers, and culture systems have been applied to develop 3D cartilage-like tissue. In this study, scaffold-free 3D micro-cartilage-like tissue was developed via microwell-mediated cell spheroid formation and 3D dynamic chondrogenic culture in a bioreactor. First, homogenous micro-cell spheroids were generated by the self-condensation of adipose tissue-derived stem cells (ADSCs) in microfabricated poly(ethylene glycol) (PEG) hydrogel microwells. Next, chondrogenic differentiation of the micro-ADSC spheroids was induced in the presence of transforming growth factor-beta 3 under dynamic 3D culture conditions using a high aspect ratio vessel bioreactor. Several hundred viable ADSC spheroids could be generated at a time from ADSC culture in PEG microwells. The 3D dynamic chondrogenic culture of ADSCs in the bioreactor facilitated the chondrogenic mRNA expression of proteins such as sox-9, runx2, osterix, type II collagen, and aggrecan, and the well deposition of glycosaminoglycan and type II collagen, which finally generated micro-cartilage-like tissue. Therefore, the hydrogel microwell arrays could be useful for efficiently deriving initial cell condensation-mediated chondrogenic differentiation, and for developing 3D cell-based micro-cartilage-like tissue with stem cells in a controlled manner.

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Authors and Affiliations

  1. Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea

    Byung-Chul Kim, Wook Byun, Il Keun Kwon & Yu-Shik Hwang

  2. Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea

    Jae Hyung Kim, Hyo Jung An & Jae-Hong Park

  3. Center for Bionics, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, 136-791, Korea

    Jin Seok Kim

Authors
  1. Byung-Chul Kim
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  2. Jae Hyung Kim
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  3. Hyo Jung An
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  4. Wook Byun
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  5. Jae-Hong Park
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  6. Il Keun Kwon
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  7. Jin Seok Kim
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  8. Yu-Shik Hwang
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Correspondence to Jin Seok Kim or Yu-Shik Hwang.

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Kim, BC., Kim, J.H., An, H.J. et al. Microwell-mediated micro cartilage-like tissue formation of adipose-derived stem cell. Macromol. Res. 22, 287–296 (2014). https://doi.org/10.1007/s13233-014-2044-7

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  • DOI: https://doi.org/10.1007/s13233-014-2044-7

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