Abstract
Dimethylsulfide (DMS), a climatically important gas generated by dimethylsulfoniopropionate (DMSP) degradation, plays an important role in the global sulfur cycle and affects the global climate. Marine bacteria are the primary mediators of DMSP degradation and DMS production. Here, we present the complete genome sequence of Rhodococcus sp. NJ-530, isolated from Antarctic sea ice, which utilizes DMSP as a sole carbon and energy source, degrading DMSP into DMS. The genome of strain NJ-530 consists of 7371 protein-coding sequences (CDSs) with 54 tRNA genes and 15 rRNA operons as 5S-16S-23S rRNA. The strain has one circular chromosome of 6,408,544 bp with 6331 CDSs and 62.41% GC content. Genomic annotation revealed that Rhodococcus sp. NJ-530 may have a DMSP cleavage gene cluster, including dddD, dddB and dddC, suggesting the existence of the DddD-type DMSP cleavage pathway. The complete genome sequence of Rhodococcus sp. NJ-530 will provide useful information for better understanding of the molecular mechanism underlying marine DMSP degradation and Antarctic DMS production.
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Acknowledgements
This work was supported by the National Key Research and Development Program of China (2018YFD0901103), China Ocean Mineral Resources R&D Association (DY135-B2-14), Open Research Fund of State Key Laboratory of Biological Fermentation Engineering of Beer (K201801), National Key Research and Development Program of China (2018YFD0900705), Natural Science Foundation of China (41576187), Natural Science Foundation of Shandong (ZR2019BD023), Key Research and Development Program of Shandong Province (2018GHY115034) and Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources (NBHY-2017-P2).
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Qu, C., Wang, W., Dong, J. et al. Complete genome sequence of Rhodococcus sp. NJ-530, a DMSP-degrading actinobacterium isolated from Antarctic sea ice. 3 Biotech 9, 363 (2019). https://doi.org/10.1007/s13205-019-1889-z
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DOI: https://doi.org/10.1007/s13205-019-1889-z