Abstract
To systematically study multi-stage countercurrent process for Antarctic krill protein extracting and to optimize the multi-stage countercurrent technology, the solubility of Antarctic krill proteins after multi-step dissolution was explored firstly; multi-step extraction was investigated; and then multi-stage countercurrent system for protein extraction was carried out. In single step extraction, krill-to-water ratio and pH were chosen as 1:10 and 12.5 respectively, in order to extract more protein. In the multi-step dissolution process, the protein solubility of aqueous solution at pH 12.5 was 33.0 ± 0.8 mg/mL. Multi-step cross-flow processing testified the feasibility of multi-stage countercurrent assumption. Three-stage countercurrent method using krill-to-water ratio 1:10 extracted, 95.1 ± 0.6% protein from krill, where almost the same water as previous works. The total recovery yield of 67.9 ± 1.6% was achieved after precipitation at pH 4.5.
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References
Adu-Peasah SP, Diosady LL, Rubin LJ (1993) A multistage hydrocyclone/stirred-tank system for countercurrent extraction of canola oil. J Am Oil Chem Soc 70:755–762
AOAC (Association of Official Analytical Chemists) (2012) Official methods of analysis of AOAC international, 19th edn. AOAC, Washington DC
Barac MB, Pesic MB, Stanojevic SP, Kostic AZ, Bivolarevic V (2015) Comparative study of the functional properties of three legume seed isolates: adzuki, pea and soy bean. J Food Sci Tech 52:2779–2787
Chen YC, Jaczynski J (2007) Gelation of protein recovered from whole Antarctic krill (Euphausia superba) by isoelectric solubilization/precipitation as affected by functional additives. J Agric Food Chem 55:1814–1822
Chen YC, Tou JC, Jaczynski J (2009) Amino acid and mineral composition of protein and other components and their recovery yields from whole Antarctic krill (Euphausia superba) using isoelectric solubilization/precipitation. J Food Sci 74:H31–H39
Gigliotti JC, Jaczynski J, Tou JC (2008) Determination of the nutritional value, protein quality and safety of krill protein concentrate isolated using an isoelectric solubilization/precipitation technique. Food Chem 111:209–214
Jafarpour A, Gorczyca EM (2009) Characteristics of sarcoplasmic proteins and their interaction with surimi and kamaboko gel. J Food Sci 74:N16–N22
Lestari D, Mulder W, Sanders J (2010) Improving Jatropha curcasseed protein recovery by using counter current multistage extraction. Biochem Eng J 50:16–23
Li K, Shen H, Li B, Wang H, Luo Y (2014) Changes in physiochemical properties of water-soluble proteins from crucian carp (Carassius auratus) during heat treatment. J Food Sci Tech 51:1396–1400
Moure A, Franco D, Sineiro J, Domínguez H, Núñez MJ (2003) Simulation of multistage extraction of antioxidants from Chilean hazelnut (Gevuina avellana) hulls. J Am Oil Chem Soc 80:389–396
Nayak R, Kenney PB, Slider S (1996) Protein extractability of turkey breast and thigh muscle with varying sodium chloride solutions as affected by calcium, magnesium and zinc chloride. J Food Sci 61:1149–1154
Ochiai Y, Chau-Jen C (2000) Myosin ATPase. In: Haard NF, Simpson BK (eds) Seafood enzymes: utilization and influence on postharvest seafood quality. Marcel Dekker, Basel, pp 69–90
Qi XM, Liao E, Wang L, Lin H, Xue CH (2016) Extracting protein from antarctic krill (euphausia superba). J Aquat Food Prod T 25:597–606
Shahidi F (1994) Seafood proteins and preparation of protein concentrates. In: Shahidi F, Botta JR (eds) Seafoods: chemistry, processing technology and quality. Springerlink, Berlin, pp 3–9
Suzuki T, Shibata N (1990) The utilization of Antarctic krill for human food. Food Rev Int 6:119–147
Takahata S, Suemura M, Noguchi M, Ohdan K (1974) US Patent No. 3844723. US Patent and Trademark Office, Washington DC
Taskaya L, Chen YC, Jaczynski J (2009) Functional properties of proteins recovered from silver carp (Hypophthalmichthys molitrix) by isoelectric solubilization/precipitation. LWT-Food Sci Technol 42:1082–1089
Tou JC, Jaczynski J, Chen YC (2007) Krill for human consumption: nutritional value and potential health benefits. Nutr Rev 65:63–77
Vareltzis PK, Undeland I (2012) Protein isolation from blue mussels (Mytilus edulis) using an acid and alkaline solubilisation technique—process characteristics and functionality of the isolates. J Sci Food Agric 92:3055–3064
Vojdani F (1996) Solubility. In: Hall GM (ed) Methods of testing protein functionality. Blackie Academic & Professional an imprint of Chapman & Hall, London, pp 9–60
Walker JM (2002) SDS polyacrylamide gel electrophoresis of proteins. In: Walker JM (ed) The protein protocols handbook, 2nd edn. Humana Press, New York, pp 61–68
Wang QE, Ma S, Fu B, Lee FS, Wang X (2004) Development of multi-stage countercurrent extraction technology for the extraction of glycyrrhizic acid (GA) from licorice (Glycyrrhiza uralensis Fisch). Biochem Eng J 21:285–292
Wang LZ, Xue CH, Wang YM, Yang B (2011) Extraction of proteins with low fluoride level from Antarctic krill (Euphausia superba) and their composition analysis. J Agric Food Chem 59:6108–6112
Yu CH, Chen J, Xiong YK, Li XX, Dai XY, Shi CC (2012) Optimization of multi-stage countercurrent extraction of antioxidants from Ginkgo biloba L. leaves. Food Bioprod Process 90:95–101
Acknowledgements
This study was supported by the Key Research and Development Project of Shandong Province (No. 2015GSF115005), Huimin Special Fund of Qingdao Municipal achievement transformation plan (No.15-9-2-120-NSH), and the Natural Science Foundation of China project (No. 31101380).
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Qi, X., Liao, E., Zhao, K. et al. Multi-stage countercurrent process for extracting protein from Antarctic Krill (Euphausia superba). J Food Sci Technol 55, 4450–4457 (2018). https://doi.org/10.1007/s13197-018-3368-7
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DOI: https://doi.org/10.1007/s13197-018-3368-7