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Optimisation of gelatin extraction from Unicorn leatherjacket (Aluterus monoceros) skin waste: response surface approach

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Abstract

Physical properties of gelatin extracted from Unicorn leatherjacket (Aluterus monoceros) skin, which is generated as a waste from fish processing industries, were optimised using Response Surface Methodology (RSM). A Box-Behnken design was used to study the combined effects of three independent variables, namely phosphoric acid (H3PO4) concentration (0.15–0.25 M), extraction temperature (40–50 °C) and extraction time (4–12 h) on different responses like yield, gel strength and melting point of gelatin. The optimum conditions derived by RSM for the yield (10.58%) were 0.2 M H3PO4 for 9.01 h of extraction time and hot water extraction of 45.83 °C. The maximum achieved gel strength and melting point was 138.54 g and 22.61 °C respectively. Extraction time was found to be most influencing variable and had a positive coefficient on yield and negative coefficient on gel strength and melting point. The results indicated that Unicorn leatherjacket skins can be a source of gelatin having mild gel strength and melting point.

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References

  • Aewsiri T, Benjakul S, Visessanguan W, Tanaka M (2008) Chemical compositions and functional properties of gelatin from pre-cooked tuna fin. Int J Food Sci Technol 43(4):685–693

    Article  CAS  Google Scholar 

  • Ahmad M, Benjakul S (2011) Characteristics of gelatin from the skin of Unicorn leatherjacket (Aluterus monoceros) as influenced by acid pretreatment and extraction time. Food Hydrocolloids 25(3):381–388

    Article  CAS  Google Scholar 

  • Arnesen JA, Gildberg A (2007) Extraction and characterisation of gelatine from Atlantic salmon (Salmo salar) skin. Bioresour Technol 98:53–57

    Article  CAS  Google Scholar 

  • Babin H, Dickinson E (2001) Influence of transglutaminase treatment on the thermoreversible gelation of gelatin. Food Hydrocolloids 15(3):271–276

    Article  CAS  Google Scholar 

  • Benjakul S, Oungbho K, Visessanguan W, Thiansilakul Y, Roytrakul S (2009) Characteristics of gelatin from the skins of bigeye snapper, Priacanthus tayenus and Priacanthus macracanthus. Food Chem 116:445–451

    Article  CAS  Google Scholar 

  • Box G, Behnken D (1960) Some new three level designs for the study of quantitative variables. Technometrics 2:455–475

    Article  Google Scholar 

  • Cho SM, Kwak KS, Park DC, Gu YS, Ji CI, Jang DH (2004) Processing optimization and functional properties of gelatin from shark (Isurus oxyrinchus) cartilage. Food Hydrocolloids 18:573–579

    Article  CAS  Google Scholar 

  • Cho SM, Gu YS, Kim SB (2005) Extracting optimization and physical properties of yellowfin tuna (Thunnus albacares) skin gelatin compared to mammalian gelatins. Food Hydrocolloids 19:221–229

    Article  CAS  Google Scholar 

  • Choi SS, Regenstein JM (2000) Physicochemical and sensory characteristics of fish gelatine. J Food Sci 65:194–199

    Article  CAS  Google Scholar 

  • Duan R, Zhang JJ, Xing FF, Konno K, Xu XJ (2011) Study on the properties of gelatins from skin of carp (Cyprinus carpio) caught in winter and summer season. Food Hydrocolloids 25:368–373

    Article  CAS  Google Scholar 

  • Gilsenan PM, Ross-Murphy SB (2000) Viscoelasticity of thermoreversible gelatin gels from mammalian and piscine collagen. J Rheology 44:871–882

    Article  CAS  Google Scholar 

  • Gomez-Guillen MC, Turnayb J, Ferandez-Diaz MD, Ulmo N, Lizarbe MA, Monteroa P (2002) Structural and physical properties of gelatin extracted from different marine species: a comparative study. Food Hydrocolloids 16:25–34

    Article  CAS  Google Scholar 

  • Gómez-Guillén MC, Giménez B, López-Caballero ME, Montero MP (2011) Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocolloids 25:1813–1827

    Article  Google Scholar 

  • Grossman S, Bergman, M (1992) Process for the production of gelatin from fish skins. US patent 5,093,474

  • Gudmundsson M, Hafsteinsson H (1997) Gelatin from cod skins as affected by chemical treatments. J Food Sci 62(1):37–39

    Article  CAS  Google Scholar 

  • Holzer D (1996) Gelatin production. US patent 5,484,888

  • Jamilah B, Harvinder KG (2002) Properties of gelatins from skins of fish—black tilapia (Oreochromis mossambicus) and red tilapia (Oreochromis nilotica). Food Chem 77(1):81–84

    Article  CAS  Google Scholar 

  • Japanese Standard Association (J.S.A.) (1996) Japanese industrial standard animal glues and gelatins. JIS K 6503, Japan

  • Jongjareonrak A, Benjakul S, Visessanguan W, Tanaka M (2006) Skin gelatin from bigeye snapper and brownstripe red snapper: chemical compositions and effect of microbial transglutaminase on gel properties. Food Hydrocolloids 20(4):1216–1222

    Article  CAS  Google Scholar 

  • Jongjareonrak A, Rawdkuen S, Chaijan M, Benjakul S, Osako K, Tanaka M (2010) Chemical compositions and characterisation of skin gelatin from farmed giant catfish (Pangasianodon gigas). LWT - Food Sci Technol 43:161–165

    Article  CAS  Google Scholar 

  • Karim AA, Bhat R (2009) Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocolloids 23:563–576

    Article  CAS  Google Scholar 

  • Kasankala LM, Xue Y, Yao W, Hong SD, He Q (2007) Optimization of gelatine extraction from grass carp (Catenopharyngodon idella) fish skin by response surface methodology. Bioresour Technol 98:3338–3343

    Article  CAS  Google Scholar 

  • Kittiphattanabawon P, Benjakul S, Visessanguan W, Nagai T, Tanaka M (2005) Characterization of acid soluble collagen from skin and bone of bigeye snapper (Priacanthus tayenus). Food Chem 89:363–372

    Article  CAS  Google Scholar 

  • Kolodziejska I, Kaczorowski K, Piotrowska B, Sadowska M (2004) Modification of the properties of gelatin from skins of Baltic cod (Gadus morhua) with transglutaminase. Food Chem 86(2):203–209

    Article  CAS  Google Scholar 

  • Ledward DA (1986) Gelation of gelatin. In: Mitchell JR, Ledward DA (eds) Functional properties of food macromolecules. Elsevier Applied Science Publishers, London, pp 171–201

    Google Scholar 

  • Mohtar NF, Perera C, Quek SY (2010) Optimisation of gelatine extraction from hoki (Macruronus novaezelandiae) skins and measurement of gel strength and SDS-PAGE. Food Chem 122:307–313

    Article  CAS  Google Scholar 

  • Montero P, Gomez-Guillen MC (2000) Extracting conditions for megrim (Lepidorhombus boscii) skin collagen affect functional properties of the resulting gelatin. J Food Sci 65(3):434–438

    Article  CAS  Google Scholar 

  • Muyonga JH, Cole CGB, Duodub KG (2004) Extraction and physicochemical characterisation of Nile perch (Lates niloticus) skin and bone gelatine. Food Hydrocolloids 8:581–592

    Article  Google Scholar 

  • Normand V, Muller S, Ravey JC, Parker A (2000) Gelation kinetics of gelatin: a master curve and network modelling. Macromolecules 33:1063–1071

    Article  CAS  Google Scholar 

  • Pranoto Y, Lee CM, Park HJ (2007) Characterizations of fish gelatin films added with gellan and k-carrageenan. LWT J Food Sci Technol 40(5):766–774

    Article  CAS  Google Scholar 

  • Rustard T (2003) Utilization of marine by-products. Electronic J Envt Agric Food Chem 2:458–463

    Google Scholar 

  • Sadowska M, Kolodziejska I, Niecikowska C (2003) Isolation of collagen from the skins of Baltic cod (Gadus morhua). Food Chem 81:257–262

    Article  CAS  Google Scholar 

  • Songchotikunpan P, Tattiyakul J, Supaphol P (2008) Extraction and electrospinning of gelatin from fish skin. Int J Biol Macromol 42:247–255

    Article  CAS  Google Scholar 

  • Tabarestani SH, Maghsoudlou Y, Motamedzadegan A, Sadeghi Mahoonak AR (2010) Optimization of physico-chemical properties of gelatin extracted from fish skin of rainbow trout (Onchorhynchus mykiss). Bioresour Technol 101(15):6207–6214

    Article  CAS  Google Scholar 

  • Yang H, Wang Y, Jiang M, Oh J, Herring J, Zhou P (2007) 2-Step optimization of the extraction and subsequent physical properties of channel catfish (Ictalurus punctatus) skin gelatin. J Food Sci 72(4):c188–c195

    Article  CAS  Google Scholar 

  • Zhou P, Regenstein JM (2004) Optimization of extraction conditions for pollock skin gelatin. J Food Sci 69(5):393–398

    Google Scholar 

  • Zhou P, Regenstein JM (2007) Comparison of water gel desserts from fish skin and pork gelatins using instrumental measurements. J Food Sci 72:C196–C201

    Article  Google Scholar 

  • Zhou P, Mulvaney SJ, Regenstein JM (2006) Properties of Alaska pollock skin gelatin: a comparison with Tilapia and pork skin gelatins. J Food Sci 71:313–321

    Article  Google Scholar 

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Acknowledgments

The authors thank Dr. W.S. Lakra, Director of Central Institute of Fisheries Education for providing all the facilities for this work.

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Authors and Affiliations

  1. Fishery Resources, Harvest and Post Harvest Management (FRHPHM) Division, Central Institute of Fisheries Education (CIFE), Mumbai, 400061, India

    Mandakini Devi Hanjabam, Sathish Kumar Kannaiyan, Gaihiamngam Kamei, Jitender Kumar Jakhar, Mithlesh Kumar Chouksey & Venkateshwarlu Gudipati

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  1. Mandakini Devi Hanjabam
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  2. Sathish Kumar Kannaiyan
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  3. Gaihiamngam Kamei
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  4. Jitender Kumar Jakhar
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  5. Mithlesh Kumar Chouksey
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  6. Venkateshwarlu Gudipati
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Correspondence to Mandakini Devi Hanjabam.

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Hanjabam, M.D., Kannaiyan, S.K., Kamei, G. et al. Optimisation of gelatin extraction from Unicorn leatherjacket (Aluterus monoceros) skin waste: response surface approach. J Food Sci Technol 52, 976–983 (2015). https://doi.org/10.1007/s13197-013-1075-y

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  • DOI: https://doi.org/10.1007/s13197-013-1075-y

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