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Development of 13 microsatellites for Gunnison Sage-grouse (Centrocercus minimus) using next-generation shotgun sequencing and their utility in Greater Sage-grouse (Centrocercus urophasianus)

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Abstract

Gunnison Sage-grouse are an obligate sagebrush species that has experienced significant population declines and has been proposed for listing under the U.S. Endangered Species Act. In order to examine levels of connectivity among Gunnison Sage-grouse leks, we identified 13 novel microsatellite loci though next-generation shotgun sequencing, and tested them on the closely related Greater Sage-grouse. The number of alleles per locus ranged from 2 to 12. No loci were found to be linked, although 2 loci revealed significant departures from Hardy–Weinberg equilibrium or evidence of null alleles. While these microsatellites were designed for Gunnison Sage-grouse, they also work well for Greater Sage-grouse and could be used for numerous genetic questions including landscape and population genetics.

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References

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Acknowledgments

The use of any trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

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Authors and Affiliations

  1. U. S. Geological Survey, Fort Collins Science Center, 2150 Centre Ave, Building C, Fort Collins, CO, 80526, USA

    Jennifer A. Fike & Sara J. Oyler-McCance

  2. Natural Resource Ecology Lab, Colorado State University (in cooperation with U.S. Geological Survey, Fort Collins Science Center), Fort Collins, CO, 80526, USA

    Shawna J. Zimmerman

  3. Department of Biology, The University of Texas at Arlington, Box 19498, Arlington, TX, 76019-0498, USA

    Todd A. Castoe

Authors
  1. Jennifer A. Fike
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  2. Sara J. Oyler-McCance
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  3. Shawna J. Zimmerman
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  4. Todd A. Castoe
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Corresponding author

Correspondence to Jennifer A. Fike.

Appendix: Microsatellite sequences for Gunnison Sage-grouse (Centrocercus minimus)

Appendix: Microsatellite sequences for Gunnison Sage-grouse (Centrocercus minimus)

SG21

TAAAAAGCAAAAGGCAAAACAGTCACACATGCAAAAAGAACCACAACAAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAGTATAGGAAGGCTAAGTGCCTTGTCCTACCTCCTGCAGGAGATGGACAGGAAGGAGCGCAGAGCAAATACCGAGATCTTCTTCACCCCAGCCTGCACTCTGCTTGTGATGTTCCCTTTGCCCTTGCAGTACTCTGTAACCTGCCTTTTGCTTTTTA

SG23

CCCAGTCACAGCCCAGAAGCAGTTAAAGCAAGGAGGTGTGATGACTGCAATGAATGGATGGATGGATGGATGGATGGATGGATGGATGGATGATTTTAAAAGGCACCTCATGTCACCAACTTTGCAGCTCTTTGGTTCCAGGTTTCATCATGAAACCCTTCAGAGACATTGAGCTGCCTGTAGCATCCTCCCTTTCTCATCTGCCTGTCCTGGGGGCAGCTGAAGGACACACTTGGAGAAGGGAAGCTCCCAGCCCCACGGCTACCCCAGGTACCTTGGAAGAGGGCAGGCTGTGCGCAGGTGATAAACGATTGCAAAGCAGTCCATTAATTCAGGAGCAAAA

SG26

TTTCTTTTACAATGAAGTGAAATTGGCCAGAAATTTAGGTGTGGTTTTGTTTGTTTATTATTATTATTATTATTATTATTATTATTATTATTTTAAATATGAAAAGTAACAGTATTGCTGTGGTAAGGGGTTTCAGATTGCTTAAAGAACTGTTA

SG27

TGTTTTTGTTTGTGTTGAACTCTTCACTGTCTAAAGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAGAGAAAGATGAGCTGAAATCCTCGGAGGTTCCTAGGGAGTGAGAGAAGAAGCACCAAGGCTATGTTTCTTCACAAACAAAAACA

SG28

TATCCCTACCTCAGCTTAAGGACTTATTAACTTAGTAACTTAAGGACACTTATACCACAGGGGAAGGACAGACTGGCGTCGTACAACAACACAGGTCATTAGCACACACACACACACACACACACACACACACAGAGGATACATCTTGGCCCTGTGTGTTTCCTCAGGGCAATGGAAAAGCAGAGGTAGGGATA

SG29

TAGAAGAAACAAGACAGTTGTATTCAGAGTTAAGGGGCTTAGGGTTTTAATGGAGAAAAATATATACACACACACACACACACACACACACACACTTTAGACATTGCTAGCTTCAGCCACTAAATAAAATAAGTTGTGTCCCCTGCCCAACTTAGTTAACTCTGAATACAACTGTCTTGTTTCTTCT

SG30

TGTTTTATTAAGTGCCTTGGTGTGGCCTTGACTCAGCTTTACTGAAACAAATTCTTGGCCTGTGAGAGAGCAAGTGAAAAGTCAGGCAGAATAAGAAAAGTAAATAAATAAATAAATAAATAAATAAATAAATAAATAAAAGGTTAGTGGGCTCGTGGGCTCATGACAGTTAGCAATTCAAGAGAAAAAATACTGAACAAAAAGGCACTTAATAAAACA

SG31

TTGTCACAGAAAATCATTAAATTGATGCAAGAACCGTTGTTTCTTCTGCCTTTTGAGAAAAGAAACACAAATTCAGATGAGATTTTACCACTGAATGAAGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGGATGAAAGGACATGACAACTGAACAGGTTTATTATTCAGGCAGTTTATTTCCAGAAA

SG33

TATTTATTTAAGTAATAGAGTGGAGTAACATTCTCTAGCTTCCCAGTGAATGAGCGTCTTTTCTACTGTGGGTCTTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTACATAGCTTCATATGGGCAATAAGAAGGGTTACACCTCAGTCTCCACCCTATTACTTAAATAAATA

SG34

TAAATGCATATAATATCTCTGAGATCAAGAAGATAACAGGAGGAATCCTCATCCCAGGAGAAGTCTTCAATTCTGGCCATTGTGTTTTTATTATTATTATTATTATTATTATTATTATTATTATACTGCAGATTAGATTTGGATTTCTCTCTATAAAGTCTTCTACAACTTTCAGAGATATTATATGCATTTA

SG36

TCCCACCTATTCCAGACATTTTGGGAGCCAGCTGAATTGGTGAAGCATTTTGTTTTTTATTTGGCATATGACATCCATCCCTTTGTATTTGCCCACAGTTTGTGTGGGGACTTTCATTTCTCCAAGGCAAAGCAGCTCTGGCAGAAGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGAAGAACTGGGTGGTTGGATGGACATGTGGATTGATGGATGGGTGGATAGGTGGGA

SG38

TGTTCCAATCAGTTCAGCAATGGTAGGTGATGGCCTTGTAGTAGGAAAGTAAGAGTCCTGTCCAAAAACAGAAGTTAAGCATCAGAAAGAGCTAAACACACACACACACACACACACACACACACACACACACACACACTGTAAAAGCTATTCTCAAACCTCAGTCCCAAGAGGCTCAGCAACATTTCTATCCAGAACTGATTGGAACA

SG39

TATATTAAGGTAAAGCGTACTGTTTCTCAACTGCAATTCTTCAGTTTCTTGAGTAGCAGAAAGTCTGAATGCTGGAGAACCTGACGGCAGGATGCATCAAATAAATCGTATTACTCATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGAAGCAGTGGGGGGGTGGTGTGGGGGAGCAAACAGTACGCTTTACCTTAATATA

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Fike, J.A., Oyler-McCance, S.J., Zimmerman, S.J. et al. Development of 13 microsatellites for Gunnison Sage-grouse (Centrocercus minimus) using next-generation shotgun sequencing and their utility in Greater Sage-grouse (Centrocercus urophasianus). Conservation Genet Resour 7, 211–214 (2015). https://doi.org/10.1007/s12686-014-0336-z

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  • DOI: https://doi.org/10.1007/s12686-014-0336-z

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