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Development and characterization of 15 novel microsatellite markers for the freshwater fish Galaxias platei

  • Microsatellite Letters
  • Published:
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Abstract

Galaxias platei is a fish species restricted to lakes and rivers from Patagonia. In this study, 15 novel microsatellite loci were developed and characterized for 64 individuals of G. platei collected from a Patagonian lake. Eight loci were polymorphic and seven were monomorphic. The number of alleles in the polymorphic loci ranged from 3 to 6, and the expected and observed heterozygosities ranged from 0.08 to 0.68, and 0.08 to 0.62, respectively. These new markers are being used in an ongoing landscape genetics study on G. platei.

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Acknowledgments

We thank Jorge González for assistance collecting samples. Funding for this research was provided by an NSERC to DER. DNA sequence data were generated on an Illumina MiSeq sequencer acquired with the support of a generous bequest from Elizabeth A. Nielsen to the Marine Gene Probe Laboratory.

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Authors and Affiliations

  1. Department of Biology, Dalhousie University, Halifax, NS, Canada

    Ivan Vera-Escalona, Ian G. Paterson & Daniel E. Ruzzante

  2. Laboratorio de Ecología y Conservación de Peces, Centro EULA, Universidad de Concepción, Concepción, Chile

    Evelyn Habit

Authors
  1. Ivan Vera-Escalona
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  2. Ian G. Paterson
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  3. Evelyn Habit
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  4. Daniel E. Ruzzante
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Correspondence to Ivan Vera-Escalona.

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Vera-Escalona, I., Paterson, I.G., Habit, E. et al. Development and characterization of 15 novel microsatellite markers for the freshwater fish Galaxias platei . Conservation Genet Resour 6, 899–901 (2014). https://doi.org/10.1007/s12686-014-0236-2

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  • DOI: https://doi.org/10.1007/s12686-014-0236-2

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