Skip to main content
SpringerLink
Log in

Isolation and characterization of eight polymorphic microsatellite loci for Natterer’s bat, Myotis nattereri (Vespertilionidae, Chiroptera)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Eight new microsatellite loci were isolated and characterized for the Natterer’s bat Myotis nattereri from a microsatellite-enriched genomic library. The usefulness of these markers was assessed by screening a sample comprising 100 specimens collected from throughout the species range in Europe. Both moderately and highly polymorphic loci were identified with 3–17 alleles segregating per locus (mean 8.1 SE ± 0.048). No evidence for departure from HWE or linkage disequilibrium among loci was observed. These markers will provide a valuable addition to the molecular toolbox currently available for studies of population genetic structure, parentage and social organisation of M. nattereri and related species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Boston ESM, Puechmaille S, Buckley DJ, Lundy MG, Scott DD, Prodöhl P, Montgomery WI, Teeling EC (2012) Empirical assessment of non-invasive population genetics in bats: comparison of DNA quality from faecal and tissue samples. Acta Chiropterol 14:45–52

    Article  Google Scholar 

  • Castella V, Ruedi M, Excoffier L (2001) Contrasted patterns of mitochondrial and nuclear structure among nursery colonies of the bat Myotis myotis. J Evol Biol 14:708–720

    Article  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA Loci. Meth Enzymol 395:202–222

    Google Scholar 

  • Jan C, Dawson DA, Altringham JD, Burke T, Butlin RK (2012) Development of conserved microsatellite markers of high cross species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Res 12:532–548

    Article  CAS  Google Scholar 

  • Mitchell-Jones AJ, Amori G, Bogdanowicz W, Kryštufek B, Reijnders PJH, Spitzenberger F, Stubbe M, Thissen JBM, Vohralík V, Zima J (1999) The Atlas of European Mammals. T & AD Poyser, London. p. 110–111, 140–141

  • Rivers NM, Butlin RK, Altringham JD (2005) Genetic population structure of Natterer's bats explained by mating at swarming sites and philopatry. Mol Ecol 14:4299–4312

    Google Scholar 

  • Rossiter S, Jones G, Ransome R, Barratt E (2001) Outbreeding increases offspring survival in wild greater horseshoe bats (Rhinolophus ferrumequinum). P R Soc Lond B 268:1055

    Article  CAS  Google Scholar 

  • Rousset F (2008) Genepop′007: a complete re-implementation of the Genepop software for windows and linux. Mol Ecol Res 8:103–106

    Article  Google Scholar 

  • Taggart JB, Hynes RA, Prodöhl PA, Ferguson A (1992) A simplified protocol for routine total DNA isolation from salmonid fishes. J Fish Biol 40:963–965

    Google Scholar 

Download references

Acknowledgments

Bats were caught under licence from the National Parks and Wildlife Service (NPWS), Ireland (Licence No.74 C/2008) and the Northern Ireland Environment Agency (Licence No. TSA/12/08). This study was funded by a research award by the NPWS, to E.C. Teeling, I.W. Montgomery and P. Prodöhl. David D. Scott was supported via a PhD studentship from the NPWS.

Author information

Authors and Affiliations

  1. Centre for Irish Bat Research, Medical and Biological Centre, Queen’s University Belfast, 97 Lisburn Rd, Belfast, N. Ireland, United Kingtom

    David D. Scott, Seán Fitzpatrick, Deborah A. Bailie, Mathieu G. Lundy, W. Ian Montgomery & Paulo A. Prodöhl

  2. Centre for Irish Bat Research, Science Centre West, University College Dublin, Belfield, Dublin 4, Ireland

    Emma S. M. Boston, Daniel J. Buckley & Emma C. Teeling

Authors
  1. David D. Scott
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seán Fitzpatrick
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Deborah A. Bailie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Emma S. M. Boston
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mathieu G. Lundy
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Daniel J. Buckley
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Emma C. Teeling
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. W. Ian Montgomery
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Paulo A. Prodöhl
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Paulo A. Prodöhl.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scott, D.D., Fitzpatrick, S., Bailie, D.A. et al. Isolation and characterization of eight polymorphic microsatellite loci for Natterer’s bat, Myotis nattereri (Vespertilionidae, Chiroptera). Conservation Genet Resour 5, 643–645 (2013). https://doi.org/10.1007/s12686-013-9871-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-013-9871-2

Keywords

Search

Navigation