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Development and characterization of 16 polymorphic microsatellite loci for the Alaska blackfish (Esociformes: Dallia pectoralis)

  • Microsatellite Letters
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Abstract

Blackfishes (Esociformes: Esocidae: Dallia), small fishes with relictual distributions, are unique in being the only primary freshwater fish genus endemic to Beringia. Although the number of species of Dallia is debated, disjunct populations and distinct mitochondrial divisions that predate the end of the last glacial maximum are apparent. We developed sixteen polymorphic microsatellites from the Alaska blackfish (Dallia pectoralis) to study genetic diversity in Dallia. Genotypes from two populations, Denali (n = 31) and Bethel (n = 35), demonstrated the usefulness of the loci for population-level investigation. Observed and expected heterozygosity averaged 18.6 and 19.8 % in Denali and 61.1 and 63.7 % in Bethel. Number of alleles at each locus averaged 3.50 in Denali and 9.63 in Bethel. The observed signature of variability and structuring between populations is consistent with mitochondrial data.

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References

  • Abdekrim J, Robertson B, Stanton JA, Gemmell N (2009) Fast, cost-effective development of species-specific microsatellite markers by genomic sequencing. Bio Techniques 46:185–192

    Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  PubMed Central  Google Scholar 

  • Faircloth BC (2008) Msatcommander: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94

    Article  CAS  PubMed  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

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Acknowledgments

The Alaska Experimental Program to Stimulate Competitive Research (AK EPSCoR) funded work by RLD and MAC, and 454 sequencing. MAC was also supported by a Discover Denali graduate fellowship, and an Institute of Arctic Biology summer fellowship. Additional funding was provided by the United States Geological Survey Alaska Science Center, the Department of Biology and Wildlife at the University of Alaska Fairbanks and a National Science Foundation Research Initiation Grant to JAL (for enriched library cloning and sequencing). We wish to thank Dr. Naoki Takebayashi for allowing MAC to use his computational resources. The University of Alaska Museum and the United States Fish and Wildlife Service Yukon-Kuskokwim National Wildlife Refuge provided specimens. Lee Tibbitts and Ann Riddle provided reviews of this manuscript prior to submission. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.

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Authors and Affiliations

  1. Department of Biology and Wildlife, University of Alaska Fairbanks, 101 Murie Building, 982 North Koyukuk Drive, Fairbanks, AK, 99775, USA

    Matthew A. Campbell & Rachel L. DeWilde

  2. Alaska Science Center, US Geological Survey, 4210 University Drive, Anchorage, AK, 99508, USA

    George K. Sage & Sandra L. Talbot

  3. University of Alaska Museum, 907 Yukon Drive, Fairbanks, AK, 99775, USA

    J. Andrés López

  4. School of Fisheries and Ocean Sciences, University of Alaska Fairbanks, 207A O’Neill Building, Fairbanks, AK, 99775, USA

    J. Andrés López

Authors
  1. Matthew A. Campbell
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  2. George K. Sage
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  3. Rachel L. DeWilde
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  4. J. Andrés López
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  5. Sandra L. Talbot
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Corresponding author

Correspondence to Matthew A. Campbell.

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Campbell, M.A., Sage, G.K., DeWilde, R.L. et al. Development and characterization of 16 polymorphic microsatellite loci for the Alaska blackfish (Esociformes: Dallia pectoralis). Conservation Genet Resour 6, 349–351 (2014). https://doi.org/10.1007/s12686-013-0091-6

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  • DOI: https://doi.org/10.1007/s12686-013-0091-6

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