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Isolation and characterization of polymorphic microsatellite DNA markers for an endangered freshwater fish in Korea, the bull-head torrent catfish Liobagrus obesus

  • Microsatellite Letters
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Abstract

The bull-head torrent catfish Liobagrus obesus (family Amblycipitidae) is an endangered freshwater fish in Korea. There has been a rapid decline in its population, caused by anthropogenic activities. This may also lead to reduced genetic diversity, which can significantly affect survival. Thus, molecular markers are important to assess the level of genetic variation. We developed 12 microsatellite markers that yielded an average of 7.83 alleles per locus. The average observed and expected heterozygosities were 0.663 and 0.699, respectively. This study is valuable for protecting the endangered species L. obesus; investigations of genetic diversity and population structure will provide useful information for conservation measures.

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References

  • Hamilton MB, Pincus EL, Di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction and genomic DNA libraries enriched for microsatellite. Biotechniques 27:500–507

    CAS  PubMed  Google Scholar 

  • Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655

    CAS  PubMed  Google Scholar 

  • Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (1998) Primer3. http://www.genome.wi.mit.edu/genome_software/other/primer3.html

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation; a review. Mol Ecol 11:1–16

    CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the Soonchunhyang University Research Fund.

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Authors and Affiliations

  1. Department of Life Science and Biotechnology, Soonchunhyang University, 646, Sinchang-myeon, Asan-si, Chungcheongnam-do, 336-745, Korea

    Hyo-Ryeon Lee, Keun-Sik Kim & In-Chul Bang

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  1. Hyo-Ryeon Lee
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  2. Keun-Sik Kim
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  3. In-Chul Bang
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Correspondence to In-Chul Bang.

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Lee, HR., Kim, KS. & Bang, IC. Isolation and characterization of polymorphic microsatellite DNA markers for an endangered freshwater fish in Korea, the bull-head torrent catfish Liobagrus obesus . Conservation Genet Resour 6, 241–243 (2014). https://doi.org/10.1007/s12686-013-0070-y

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  • DOI: https://doi.org/10.1007/s12686-013-0070-y

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