Abstract
Corydalis hirtipes is an endangered endemic species of Korea. The geographic distribution range of this species is very restricted, and currently only one locality with a low number of individuals is known. We isolated and characterized eight microsatellite loci in this species. The number of alleles ranged from 4 to 19, observed heterozygosity from 0.39 to 0.88, and expected heterozygosity from 0.43 to 0.88. The markers described here will be useful for investing the genetic diversity, genetic structure, and gene flow of C. hirtipes, and the genetic findings would contribute to the establishment of effective conservation measures for this species.
References
Bernardos S, Amado A, Amich F (2006) The narrow endemic Scrophularia valdesii Ortega-Olivencia & Devesa (Scrophulariaceae) in the Iberian Peninsula: an evaluation of its conservation status. Biodivers Conserv 15:4027–4043. doi:10.1007/s10531-005-3017-0
Bizoux JP, Brevers F, Meerts P, Graitson E, Mahy G (2004) Ecology and conservation of Belgian population of Viola calaminaria, a metallophyte with a restricted geographic distribution. Belg J Bot 137:97–104
Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486
Lande R (1988) Genetic and demography in biological conservation. Science 241:1455–1460
Lian C, Hogetsu T (2002) Development of microsatellite markers in black locust (Robinia pseudoacasia) using a dual-suppression-PCR technique. Mol Ecol Notes 2:211–213. doi:10.1046/j.1471-8286.2002.00213.x-i2
Lian C, Wadud MA, Geng Q, Shimatani K, Hougetsu T (2006) An improved technique for isolating codominant compound microsatellite markers. J Plant Res 119:415–417. doi:10.1007/s10265-006-0274-2
Milligan B (1992) Plant DNA isolation. In: Hoelzel AR (ed) Molecular genetic analysis of populations: a practical approach. IRL Press, Oxford, pp 59–88
Oh BU, Jang CG (2002) Corydalis hirtipes B.U. Oh et J.G. Kim: a new species of Corydalis sect. Corydalis (Fumariaceae) from Korea. J Plant Biol 45: 237–239. doi:10.1007/BF03030366
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Schemske DW, Husband BC, Ruckelhaus MH, Goodwillie C, Parker IM, Bishop JG (1994) Evaluating approaches to the conservation of rare and endangered plants. Ecology 75:584–606. doi:10.2307/1941718
Vié JC, Hilton-Taylor C, Stuart SN (2009) Wildlife in a changing world—an analysis of the 2008 IUCN red list of threatened species. IUCN, Gland
Acknowledgments
This research was supported by the Environment Research and Technology Development Fund (S-9-2) of the Ministry of the Environment, Japan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Choi, H.J., Kaneko, S., Oh, B.U. et al. Development of eight microsatellite markers for Corydalis hirtipes (Fumariaceae), a narrow endemic species of Korea. Conservation Genet Resour 4, 407–409 (2012). https://doi.org/10.1007/s12686-011-9561-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-011-9561-x