Skip to main content

Advertisement

SpringerLink
Log in

Isolation and characterization of microsatellite markers for the endangered crab species Sesarmops intermedius

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Sesarmops intermedius, which is designated as an endangered species in Korea, has been severely threatened by habitat destruction and pollution. From this species, we isolated 9 polymorphic microsatellites, which are independent of each other and represent allele frequencies under the Hardy–Weinberg equilibrium, except for 1 locus. The number of alleles per locus ranged from 7 to 20. The expected and observed heterozygosities ranged from 0.77 to 0.96 and from 0.63 to 0.95, respectively. We also examined cross-species amplification of the 9 developed microsatellite loci in 2 other crabs designated as endangered species in Korea and 6 sesarmid crabs, which live in habitats similar to that of S. intermedius. These markers have the potential to contribute to establishing conservation plans for this endangered crab species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Excoffier L, Laval G, Schneider S (2005) Arlequin Ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  Google Scholar 

  • Hammond RL, Saccheri IJ, Ciofi C et al (1998) Isolation of microsatellite markers in animals. In: Karp A, Isaac PG, Ingram DS (eds) Molecular tools for screening biodiversity. Chapman & Hall, Weinheim, pp 279–285

    Chapter  Google Scholar 

  • Kim HS (1973) Illustrated encyclopedia of fauna and flora of Korea, vol. 14, Anomura and Brachyura. Ministry of Education, Seoul, pp 1–694 (in Korean)

    Google Scholar 

  • Rozen S, Skaletsky HJ (2000) PRIMER 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Van Oosterhout C, Hutchinson F, Wills D, Shipley P (2004) Microchecker: software for identifying and correcting genotype errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

Download references

Acknowledgments

This project was supported by a grant from the Eco-Technopia 21 Project funded by the Ministry of Environment, Korea and a grant from the Marine Biotechnology Programme funded by the Ministry of Land, Transport and Maritime Affairs of Korean government.

Author information

Authors and Affiliations

  1. School of Biological Sciences, Seoul National University, Seoul, 151-747, Korea

    Sang-kyu Lee, Seunghyun Kang & Won Kim

  2. Department of Science Education, Ewha Womans University, Seoul, 120-750, Korea

    Jongwoo Jung

Authors
  1. Sang-kyu Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jongwoo Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Seunghyun Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Won Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Won Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, Sk., Jung, J., Kang, S. et al. Isolation and characterization of microsatellite markers for the endangered crab species Sesarmops intermedius . Conservation Genet Resour 4, 183–185 (2012). https://doi.org/10.1007/s12686-011-9504-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-011-9504-6

Keywords

Search

Navigation