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Disorder of trafficking system of plasma membrane and vacuole antiporter proteins causes hypersensitive response to salinity stress in Arabidopsis Thaliana

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Abstract

Rab GTPases play an important role in regulating intracellular vesicular trafficking in eukaryotic cells. Previously, we found that Oryza sativa rice Rab11 (OsRab11) is required for the regulation of vesicular trafficking from the trans- Golgi network (TGN) to the plasma membrane (PM) and/or vacuoles. To further elucidate the relationship between vesicular trafficking and abiotic and biotic stresses, we determined OsRab11 expression levels under several environmental stress conditions. OsRab11 expression was induced by pathogens, jasmonic acid (JA), and high salt treatment. Under high salt conditions, dominant negative OsRab11(S28N) mutant plants exhibited a hypersensitive phenotype similar to that of sos1-1, whereas overexpressed-OsRab11 plants showed resistance to high salt stress. When the expression of vacuolar and PM Na+/H+ antiporter genes such as AtNHX1, AtNHX2, and AtSOS1 was examined, there was no significant difference between the wild-type and OsRab11(S28N) mutant plants. However, PM trafficking of AtSOS1-green fluorescent protein (GFP) in 35S::AtSOS1-GFP sos1-1 plants was severely impaired by T7-OsRab11(S28N) expression. Similarly, vacuolar trafficking of AtNHX2-GFP was inhibited by T7-OsRab11 (S28N) expression. These results indicate that trafficking of PM and vacuolar antiporter proteins by OsRab11 is important for high salt stress resistance.

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  1. These authors contributed equally to the work.

Authors and Affiliations

  1. Department of Molecular Genetic Biotechnology, Dong-A University, Busan, 49315, Korea

    Jee Hye Kim, Chong Chen, Hee Rang Yun, Young Byung Yi & Jae Bok Heo

  2. Department of Biotechnology, Dong-A University, Busan, 49315, Korea

    Yong-Suk Lee

  3. Asia Seed Company, Icheon, 17414, Korea

    Tae-Yun Kim

  4. Department of Bioindustry and Bioresource Engineering, Sejong University, Seoul, 05006, Korea

    Hyun Uk Kim

Authors
  1. Jee Hye Kim
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  2. Chong Chen
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  3. Hee Rang Yun
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  4. Yong-Suk Lee
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  5. Young Byung Yi
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  6. Tae-Yun Kim
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  8. Jae Bok Heo
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Correspondence to Jae Bok Heo.

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12374_2017_42_MOESM1_ESM.pptx

Disorder of Trafficking System of Plasma Membrane and Vacuole Antiporter Proteins Causes Hypersensitive Response to Salinity Stress in Arabidopsis Thaliana

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Kim, J.H., Chen, C., Yun, H.R. et al. Disorder of trafficking system of plasma membrane and vacuole antiporter proteins causes hypersensitive response to salinity stress in Arabidopsis Thaliana . J. Plant Biol. 60, 380–386 (2017). https://doi.org/10.1007/s12374-017-0042-y

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  • DOI: https://doi.org/10.1007/s12374-017-0042-y

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