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Association of IL-10 gene (−1082A>G, −819C>T and −592C>A) polymorphism and its serum level with metabolic syndrome of north Indian subjects

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Abstract

Metabolic syndrome (MetS) is an inflammatory disorder, in which various cytokines play important role in tilting balance towards disease state. Interleukin-10 (IL-10) is an important antiinflammatory cytokine, but its genetic polymorphisms and serum levels in Indian MetS subjects are unknown. Three IL-10 gene polymorphisms (−1082A >G (rs1800896), −819C >T (rs1800872) and −592C >A (rs1800871)) were genotyped with PCR-RFLP in MetS subjects (n = 384) and age/sex matched control subjects (n = 386). Serum IL-10 was measured using enzyme-linked immunosorbent assay. Serum IL-10 level was significantly low in MetS subject and significantly correlated with clinicobiochemical parameters of MetS. Of three investigated promoter polymorphisms, IL-10 –819C > T and –592C >A were significantly associated with risk of MetS. The mutant alleles −819T and −592A of IL-10 gene polymorphism were significantly higher in MetS subjects compared to controls. Of the four different haplotypes obtained, common ACC haplotype and rare GTA haplotype of IL-10 polymorphisms were associated with MetS. The mean of fasting insulin and HOMA-IR were significantly different between the genotypes of both −819 C >T and −592C >A polymorphisms of IL-10 in MetS subjects. These results suggested that polymorphisms in IL-10 gene (−819C >T and −592C >A), haplotypes (ACC and GTA) and serum level are significantly associated with risk of MetS. IL-10 −819C >T and −592C >A polymorphic variants are also significantly associated with insulin level and homeostasis model assessment-insulin resistance in north Indian MetS subjects.

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Acknowledgements

The authors are thankful to the healthy controls and patients for participating in the study. This work was potentially supported by a grant for scientific research from the Indian Council of Medical Research, New Delhi, India (grant no. 5/4/8-3/2010-NCD-II). The authors are grateful to the Director, CSIR-Central Drug Research Institute and colleagues from Endocrinology Division, CSIR-Central Drug Research Institute, Lucknow, India. We are also thankful to the Department of Physiology and Department of Pathology, King George’s Medical University, Lucknow, India, for their support in the study.

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  1. Department of Physiology, King George’s Medical University, Lucknow, 226 003, India

    AMIT KUMAR MADESHIYA, SHRADDHA SINGH & SHIPRA DWIVEDI

  2. Department of Pathology, King George’s Medical University, Lucknow, 226 003, India

    RITURAJ KONWAR

  3. Division of Endocrinology, CSIR-Central Drug Research Institute, Jankipuram Extension, Lucknow, 226 031, India

    SHANKAR MADHAV NATU

  4. Division of Clinical and Experimental Medicine, CSIR-Central Drug Research Institute, Jankipuram Extension, Lucknow, 226 031, India

    ASHIM GHATAK

  5. Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110 025, India

    RITURAJ KONWAR & ASHIM GHATAK

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[Madeshiya A. K., Singh S., Dwivedi S., Konwar R., Natu S. M. and Ghatak A. 2017 Association of IL-10 gene (−1082A >G, −819C >T and −592C >A) polymorphism and its serum level with metabolic syndrome of north Indian subjects. J. Genet. 95, xx–xx]

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MADESHIYA, A.K., SINGH, S., DWIVEDI, S. et al. Association of IL-10 gene (−1082A>G, −819C>T and −592C>A) polymorphism and its serum level with metabolic syndrome of north Indian subjects. J Genet 96, 53–64 (2017). https://doi.org/10.1007/s12041-016-0738-7

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