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5-HT7 Receptors Are Not Involved in Neuropeptide Release in Primary Cultured Rat Trigeminal Ganglion Neurons

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Abstract

Migraine is a common but complex neurological disorder. Its precise mechanisms are not fully understood. Increasing indirect evidence indicates that 5-HT7 receptors may be involved; however, their role remains unknown. Our previous in vivo study showed that selective blockade of 5-HT7 receptors caused decreased serum levels of calcitonin gene-related peptide (CGRP) in the external jugular vein following electrical stimulation of the trigeminal ganglion (TG) in an animal model of migraine. In the present study, we used an in vitro model of cultured TG cells to further investigate whether 5-HT7 receptors are directly responsible for the release of CGRP and substance P from TG neurons. We stimulated rat primary cultured TG neurons with capsaicin or potassium chloride (KCl) to mimic neurogenic inflammation, resulting in release of CGRP and substance P. 5-HT7 receptors were abundantly expressed in TG neurons. Greater than 93 % of 5-HT7 receptor-positive neurons co-expressed CGRP and 56 % co-expressed substance P. Both the capsaicin- and KCl-induced release of CGRP and substance P were unaffected by pretreatment of cultured TG cells with the selective 5-HT7 receptor agonist AS19 and antagonist SB269970. This study demonstrates for the first time that 5-HT7 receptors are abundantly co-expressed with CGRP and substance P in rat primary TG neurons and suggests that they are not responsible for the release of CGRP and substance P from cultured TG neurons evoked by capsaicin or KCl.

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Abbreviations

ANOVA:

Analysis of variance

AS19:

(2S)-(+)-5-(1,3,5-trimethylpyrazol-4-yl)-2-(dimethylamino) tetralin

BSA:

Bovine serum albumin

CGRP:

Calcitonin gene-related peptide

DMSO:

Dimethyl sulfoxide

ELISA:

Commercial enzyme-linked immunosorbent assay

HBS:

HEPES-buffered saline

HEPES:

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

KCl:

Potassium chloride

LSD:

Fisher’s least significance difference

MAP2:

Microtubule associated protein 2

PBS:

Phosphate-buffered saline

SB269970:

(2R)-1-[(3-hydroxyphenyl) sulfonyl]-2-[2-(4-methyl-1-piperidinyl) ethyl] pyrrolidine hydrochloride

SGC:

Satellite glial cells

SP:

Substance P

TG:

Trigeminal ganglion

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant number 81100830); the Science and Technology Program of Guangzhou, China (grant numbers 2012 J5100039 and 2012 J4300086); and the Medical Science and Technology Project of Guangzhou (grant number 20121A011025).

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Authors and Affiliations

  1. Department of Neurology, Guangzhou First People’s Hospital, Affiliated to Guangzhou Medical University, No. 1 Panfu Road, Guangzhou, 510180, China

    Xiaojuan Wang, Rong Hu, Ze Li & Xiaoping Pan

  2. Department of Clinical Laboratory, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, Guangzhou, 510080, China

    Jianbo Liang

  3. Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neurosciences and the Second Affiliated Hospital of Guangzhou Medical University, No. 250 Changgang Dong Road, Guangzhou, 510260, China

    Weiwen Sun

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  1. Xiaojuan Wang
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Correspondence to Xiaoping Pan.

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Wang, X., Hu, R., Liang, J. et al. 5-HT7 Receptors Are Not Involved in Neuropeptide Release in Primary Cultured Rat Trigeminal Ganglion Neurons. J Mol Neurosci 59, 251–259 (2016). https://doi.org/10.1007/s12031-016-0727-6

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  • DOI: https://doi.org/10.1007/s12031-016-0727-6

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