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Cytogenetic mechanism for the aneuploidy and mosaicism found in tetraploid Pacific oyster Crassostrea gigas (Thunberg)

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Abstract

Chromosome constitution was investigated in adult tetraploid Pacific oyster produced by blocking the first polar body of triploid eggs which were fertilized with haploid sperms. A high incidence of aneuploid and heteroploid mosaics were found among the offspring. Of 20 individuals identified, only 9 (45%) were eutetraploid which contained 40 chromosomes; 2 (10%) were aneuploid (hypotetraploid), which contained 39 and 38 chromosomes, respectively; and 9 (45%) were heteroploid mosaics. One mosaic was consisted of cells containing 40 and 39 chromosomes, respectiovely (1:1 in cell number), while the other 8 were consisted of cells containing chromosomes varying between tetraploid and triploid. It was also interesting to note that 3 mosaics even contained some diploid cells with 20 chromosomes. A certain number of cells of 2 tetraploids and 8 mosaics spread with 32–37 well-scattered and some clumped chromosomes at metaphase. The percentage of aneuploid cells with chromosomes varying between triploid and tetraploid correlated significantly with that of heteroploid mosaics cells with clumping chromosomes (P<0.05). Our findings suggested that reversion existed in both tetraploid and triploid oyster and chromosome clumping may underline the chromosome elimination in tetraploid oyster. It seems that the reversing cells, at least some of them, continuously eliminate their chromosomes until the most stable diploid state is established.

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Authors and Affiliations

  1. College of Marine Life Sciences, Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao, 266003, P. R. China

    Zhengrui Zhang, Xinglian Wang & Quanqi Zhang

  2. Aquaculture Genetics and Breeding Technology Center, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA, 23062, USA

    Standish Allen Jr.

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  1. Zhengrui Zhang
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  2. Xinglian Wang
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  3. Quanqi Zhang
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  4. Standish Allen Jr.
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Correspondence to Quanqi Zhang.

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Zhang, Z., Wang, X., Zhang, Q. et al. Cytogenetic mechanism for the aneuploidy and mosaicism found in tetraploid Pacific oyster Crassostrea gigas (Thunberg). J. Ocean Univ. China 13, 125–131 (2014). https://doi.org/10.1007/s11802-014-2318-x

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  • DOI: https://doi.org/10.1007/s11802-014-2318-x

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