Abstract
The effect of ultraviolet-B (UV-B) radiation on Antarctic phytoplankton has become an attractive ecological issue as a result of annual springtime ozone depletion. The effects of UV-B radiation on the growth and antioxidant enzymes were investigated using Antarctic sea ice microalgae Chlamydomonas sp. ICE-L as the material in this study. The results demonstrated that UV-B radiation could notably inhibit the growth, especially at high UV-B radiation intensity (70 μW cm−2). Malondialdehyde and O2 ·− content in ICE-L increased rapidly in early days (1–3 days) exposed to UV-B radiation enhancement, then decreased rapidly. In the stress of UV-B radiation enhancement, the superoxide dismutase, peroxidase and Catalase activities of 1–4 days in ICE-L were obviously higher than those in the control, and their activities became higher at high UV-B radiation intensity (70 μW cm−2). These enzymes activity of 7 days would kept stable at low UV-B radiation intensity (35 μW cm−2), but kept high level at high UV-B radiation intensity (70 μW cm−2). However, the ascorbate peroxidase activity in ICE-L kept stable under the stress of UV-B radiation enhancement. The above experimental results indicated that the antioxidant enzyme system played an important role in the adaptation of Antarctic ice microalgae under the UV-B radiation change of Antarctic ecosystems.
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Abbreviations
- APX:
-
Ascorbate peroxidase
- CAT:
-
Catalase
- DTT:
-
Dithiothreitol
- EDTA:
-
Ethylenediamine tetraacetic acid
- H2O2 :
-
Hydrogen peroxide
- MDA:
-
Malondialdehyde
- POD:
-
Peroxidase
- PVP:
-
Polyvinylpyrrolidone
- SOD:
-
Superoxide dismutase
- TBA:
-
Thiobarbituric acid
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Acknowledgments
This study was supported by the natural scientific research innovation foundation in Harbin Institute of Technology (No. 2008-067), the Key Lab of Marine Bioactive Substances, SOA (No. MBS-2007-01), the Natural Science Foundation of Shandong Province (No. Y2007D58), Science and Technology Development Project of Weihai (No. 2008087) and the National Natural Science Foundation of China (No. 40876107).
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Communicated by J. Zwiazek.
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Wang, Qf., Hou, Yh., Miao, Jl. et al. Effect of UV-B radiation on the growth and antioxidant enzymes of Antarctic sea ice microalgae Chlamydomonas sp. ICE-L. Acta Physiol Plant 31, 1097–1102 (2009). https://doi.org/10.1007/s11738-009-0271-x
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DOI: https://doi.org/10.1007/s11738-009-0271-x