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Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction

  • Experimental Research
  • Published:
Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the possible protective effect and mechanism of ginsenoside Rb1 against oxidative damage and renal interstitial fibrosis on rats with unilateral ureteral obstruction (UUO).

Methods

In total, 80 male rats were randomly divided into 4 groups, 20 in each group: the sham operated group (SOR), UUO group, UUO with ginsenoside Rb1 treatment group (treated with intraperitoneal injection of 50 mg/ kg daily) and UUO with Losartan treatment group (as the positive control, treated with 20 mg/kg by gastrogavage per day). The rats were randomly sacrifificed on day 3, 7 and 14 after surgery, respectively. The histopathologic changes of renal interstitial tissues were observed with Masson staining. The mRNA of transforming growth factor β 1 (TGF-β 1), collagen I and fibronectin were reversed transcribed and quantified by Real-time PCR. Enzyme-linked immunosorbent assay was used to quantitatively detect TGF-β 1 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. P47phox protein expression was assessed by immunohistochemistry and Western blot analysis.

Results

In the UUO model, the obstructed kidney showed typical features of progressive renal tubulointerstitial fibrosis, and the levels of TGF-β1, collagen I and fibronectin increased (P<0.05). As compared with the UUO group, ginsennoside Rb1 significantly inhibited the interstitial fibrosis including tubular injury and collagen deposition, and decreased the levels of TGF-β1 (P<0.05). Ginsenoside Rb1 also inhibited the heme oxygenase (HO-1) and 8-OHdG, two markers of oxidative stress (P<0.05). Moreover, ginsenoside Rb1 suppressed the expression of p47phox, a subunit of nicotinamide adeninedinucleotide phosphate (NADPH) oxidase (P<0.05).

Conclusion

Ginsenoside Rb1 can obviously inhibit renal interstitial fibrosis in rats with UUO, its mechanism possibly via against the oxidative damage and suppressing TGF-β1 expression.

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Authors and Affiliations

  1. Department of Nephrology, West China Hospital, Sichuan University, Chengdu, 610041, China

    Xi-sheng Xie  (谢席胜), Man Yang  (杨 满), Chuan Zuo  (左 川), Yao Deng  (邓 尧) & Jun-ming Fan  (樊均明)

  2. Department of Medicine Technology, West China School of Public Health, Sichuan University, Chengdu, 610041, China

    Heng-chuan Liu  (刘衡川)

  3. State Key Laboratory of Biotherapy of Human Disease, West China Hospital, Sichuan University, Chengdu, 610041, China

    Jun-ming Fan  (樊均明)

Authors
  1. Xi-sheng Xie  (谢席胜)
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  2. Heng-chuan Liu  (刘衡川)
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Correspondence to Jun-ming Fan  (樊均明).

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Xie, Xs., Liu, Hc., Yang, M. et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction. Chin. J. Integr. Med. 15, 133–140 (2009). https://doi.org/10.1007/s11655-009-0133-9

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  • DOI: https://doi.org/10.1007/s11655-009-0133-9

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