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New Korean isolates of Pepper mild mottle virus (PMMoV) differ in symptom severity and subcellular localization of the 126 kDa protein

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Abstract

Two isolates of Pepper mild mottle virus (PMMoV) were selected from a nationwide survey of pepper fields in South Korea in 2014 and 2015, in which Cucumber mosaic virus was also detected; the two PMMoV isolates, Sangcheong 47 (S-47, KX399390) and Jeongsong 76 (J-76, KX399389), share ~99% nucleotide and amino acid identity and are closely related to Japanese and Chinese isolates at the nucleotide level. Amino acid sequence comparisons revealed 99.73, 99.81, 98.44, and 100% identity in the ORF1, ORF2, MP, and CP, respectively, between S-47 and J-76. In addition, we generated infectious clones of S-47 and J-76, and T7 promoter driven transcripts of each inoculated to Nicotiana benthamiana produced very severe symptoms, whereas only mild symptoms developed in Capsicum annuum. Gene silencing suppressor function of 126 kDa and cytoskeleton-connected plasmodesmata localization of movement protein of S-47 and J-76 showed no difference between isolates, whereas 126 kDa of J-76 clearly formed intracellular aggregates not observed with S-47 126 kDa protein. Differences between these isolates in 126/183 kDa-related functions including subcellular localization suggest that differential interactions with host proteins may affect symptom development in C. annuum.

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Acknowledgements

This research was supported by Technology Commercialization Support Program (Project No. 113044–3), Ministry of Agriculture, Food and Rural Affairs.

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    Authors and Affiliations

    1. Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 305-764, Korea

      Sang-Hyuk Han, Jong-Seo Park, Jae-Yeong Han, Jun-Su Gong, Chan-Hwan Park, Jung-Kyu Kim, Eun-Young Seo & Hyoun-Sub Lim

    2. United States Department of Agriculture – Agricultural Research Service, University of Illinois at Urbana-Champaign, Department of Crop Sciences, Champaign, IL, 61801, USA

      Leslie L. Domier

    3. United States Department of Agriculture – Agricultural Research Service, United States National Arboretum, Floral and Nursery Plants Research Unit, 10300 Baltimore Avenue B-010A, Beltsville, MD, 20705, USA

      John Hammond

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    Sang-Hyuk Han, Jong-Seo Park and Jae-Yeong Han have contributed equally to this study.

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    Han, SH., Park, JS., Han, JY. et al. New Korean isolates of Pepper mild mottle virus (PMMoV) differ in symptom severity and subcellular localization of the 126 kDa protein. Virus Genes 53, 434–445 (2017). https://doi.org/10.1007/s11262-017-1432-4

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