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Arabidopsis thaliana NAC083 protein interacts with Mungbean yellow mosaic India virus (MYMIV) Rep protein

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Abstract

Geminiviral replication initiator protein (Rep) is a key player in geminiviral rolling circle mode of replication. However, the virus exploits various host cellular machineries for its replication. Study of these host factors is important to understand the geminiviral DNA replication in greater details. With this view, we screened for the peptides interacting with the Rep protein of a representative of geminivirus, namely, Mungbean yellow mosaic India virus (MYMIV), employing phage display technique. Through this screen, we have identified a host transcription factor, NAC083, as a potential MYMIV-Rep-binding partner. In silico docking studies also suggested possible binding of NAC083 peptide to MYMIV-Rep. We validated the interaction between MYMIV-Rep and Arabidopsis thaliana full-length NAC083 protein using in vitro pull-down assay and yeast two-hybrid analysis. NAC proteins are well-known transcription factors belonging to the largest gene families in plants. This study demonstrates for the first time the interaction of NAC083, a member of NAC transcription factor family, with MYMIV-Rep protein thereby indicating its possible role in MYMIV DNA replication.

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Acknowledgments

We thank Ms. Aditi Agarwal for her help in the docking studies. The financial assistance received from Department of Science and Technology, Government of India, in the form of Junior Research Fellowship to VSR is gratefully acknowledged.

Conflict of interest

None.

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Authors and Affiliations

  1. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110 067, India

    Geetika Suyal & Nirupam Roy Choudhury

  2. Department of Biotechnology, Faculty of Science, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi, 110 062, India

    Geetika Suyal & Saima Wajid

  3. Department of Zoology, University of Delhi, New Delhi, 110 007, India

    Vipin Singh Rana

  4. Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India

    Sunil Kumar Mukherjee

Authors
  1. Geetika Suyal
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  2. Vipin Singh Rana
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  3. Sunil Kumar Mukherjee
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  4. Saima Wajid
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  5. Nirupam Roy Choudhury
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Corresponding author

Correspondence to Nirupam Roy Choudhury.

Additional information

Geetika Suyal and Vipin Singh Rana have contributed equally to this study.

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Suyal, G., Rana, V.S., Mukherjee, S.K. et al. Arabidopsis thaliana NAC083 protein interacts with Mungbean yellow mosaic India virus (MYMIV) Rep protein. Virus Genes 48, 486–493 (2014). https://doi.org/10.1007/s11262-013-1028-6

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  • DOI: https://doi.org/10.1007/s11262-013-1028-6

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