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Molecular evidence and sequence analysis of a natural reassortant between Cucumber mosaic virus subgroup IA and II strains

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Abstract

Cucumber mosaic virus (CMV) is a tripartite RNA virus and has been divided into three subgroups, named IA, IB, and II. Some studies have found a few natural reassortants between CMV subgroups, although reassortment between CMV subgroups is infrequent. In our present work, a CMV reassortant, named CMV-Tsh, was obtained from a tomato plant. The complete sequence of CMV-Tsh genomic RNAs has been determined and analyzed. The results of sequence comparisons and phylogenetic analyses revealed that CMV-Tsh RNAs 1 and 3 are derived from one or two CMV subgroup II strain(s), while RNA2 is derived from a CMV subgroup IA strain. A PCR and restriction enzyme analysis-based method was developed to analyze the possibility of mixed infection by CMV strains of different subgroup in the CMV-Tsh-infected tomato plant. The results of the restriction enzyme analysis proved that CMV-Tsh is the unique strain in the tomato plant. Taken together, CMV-Tsh is a natural reassortant having CMV subgroup IA RNA2 and subgroup II RNAs 1 and 3.

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Acknowledgments

We thank Dr. Peter Palukaitis for kindly supplying the full-length cDNA clones of CMV Fny (pFny109, pFny209, and pFny309) and LS (pLS109, pLS209 and pLS309) strains. This work was supported by the National Science Foundation of China (30270744).

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Authors and Affiliations

  1. Institute of Bioengineering, Zhejiang Sci-Tech University, No.2 Road Xiasha, Hangzhou, Zhejiang, 310018, P.R. China

    Yanfei Chen, Jishuang Chen, Huarong Zhang, Xiangshan Tang & Zhiyou Du

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  1. Yanfei Chen
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  2. Jishuang Chen
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  3. Huarong Zhang
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  4. Xiangshan Tang
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  5. Zhiyou Du
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Corresponding author

Correspondence to Jishuang Chen.

Additional information

The GenBank Accession numbers of the sequences reported in this paper are EF202595- EF202597.

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Chen, Y., Chen, J., Zhang, H. et al. Molecular evidence and sequence analysis of a natural reassortant between Cucumber mosaic virus subgroup IA and II strains. Virus Genes 35, 405–413 (2007). https://doi.org/10.1007/s11262-007-0094-z

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