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Urine and serum ghrelin, sCD80 and sCTLA-4 levels in doxorubicin-induced experimental nephrotic syndrome

  • Nephrology - Original Paper
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Abstract

Background

Nephrotic syndrome (NS) is an immune-mediated disorder associated with hyperlipidemia. NS has been proposed to be mediated through CD80-related T cell immune response, which could be blocked using soluble cytotoxic T lymphocyte-associated s(CTLA)-4. Although ghrelin is a hormone-modulating lipid metabolism and suppressing immune system, the precise role of ghrelin in NS is not well established.

Methods

We evaluated the levels of ghrelin, soluble CD80 (sCD80) and sCTLA4 in serum and urine in doxorubicin-induced NS in rats. We also investigated the relation between their levels and the levels of serum total cholesterol (TC), triglyceride, albumin and urine protein.

Results

While urinary ghrelin levels were significantly lower in the nephrotic rats compared to the control group, serum ghrelin levels were comparable in the nephrotic and control rats. In contrast, serum and urinary sCD80 and sCTLA4 levels were higher in the nephrotic rats than the controls. The urinary ghrelin levels were negatively correlated with the levels of serum triglyceride, TC and urine protein, sCD80 and sCTLA4. The urine sCD80 levels were positively correlated with the TC, urine protein and urine sCTLA4 levels, and negatively correlated with the serum albumin. The urine sCTLA4 levels were positively correlated with the TC and urine protein levels and negatively correlated with the serum albumin levels. In regression analysis, the urine ghrelin levels significantly relate to urine sCD80 levels. Besides, hyperlipidemia in NS did not appear to be related to serum ghrelin levels.

Conclusion

Low urine ghrelin levels might be relevant to pathogenesis of doxorubicin-induced NS. The reduction in urine ghrelin levels might also be associated with increased levels of urine sCTLA4 and sCD80 which reflect proteinuria.

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Authors and Affiliations

  1. Department of Pediatric Nephrology, Faculty of Medicine, Eskisehir Osmangazi University, TR-26480, Eskisehir, Turkey

    Duygu Ozkorucu, Nuran Cetin, Nadide Melike Sav & Bilal Yildiz

Authors
  1. Duygu Ozkorucu
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  2. Nuran Cetin
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  3. Nadide Melike Sav
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  4. Bilal Yildiz
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Correspondence to Bilal Yildiz.

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Conflict of interest

Duygu Ozkorucu, Nuran Cetin, Nadide Melike Sav and Bilal Yildiz declare that they have no conflict of interest.

Human and animal rights

All applicable international, national and institutional guidelines for the care and use of animals were followed. All animal experiments were approved by the Committee for Animal Experiments of the Eskisehir Osmangazi University (Number: 32/186-1).

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Ozkorucu, D., Cetin, N., Sav, N.M. et al. Urine and serum ghrelin, sCD80 and sCTLA-4 levels in doxorubicin-induced experimental nephrotic syndrome. Int Urol Nephrol 48, 1187–1196 (2016). https://doi.org/10.1007/s11255-016-1249-4

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  • DOI: https://doi.org/10.1007/s11255-016-1249-4

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