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Identification of polymorphism in fatty acid binding protein 3 (FABP3) gene and its association with milk fat traits in riverine buffalo (Bubalus bubalis)

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Abstract

The fatty acid binding protein 3 (FABP3) gene, known to be associated with fat percentage of milk and meat in bovines, was screened among swamp and riverine buffaloes for polymorphism detection and further association with milk fat contents. An SNP g.307C > T was identified in the intron 2 (+53 exon 2) region of FABP3 gene of Indian buffaloes. The SNP identified was genotyped in 692 animals belonging to 15 riverine, swamp and hybrid (riverine × swamp) buffalo populations of diverse phenotypes and utilities, by PCR-RFLP. A marked contrast was observed between the C and T allele frequencies in three types of buffaloes. The frequency of C allele ranged from 0.67 to 0.96 in pure swamp buffalo populations, with the highest in Mizoram (0.96). Whereas the frequency of T allele was high across all the Indian riverine buffalo breeds, ranging from 0.57 to 0.96. None of the genotypes at FABP3 g.307C > T locus was found to have significant association with milk fat and other production traits in Mehsana dairy buffalo breed. Our study revealed marked differences in the allele frequencies between riverine and swamp buffaloes at FABP3 g.307C > T locus, without any significant association with different milk traits in riverine buffaloes.

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Acknowledgments

Authors wish to record their thanks to the Director, National Bureau of Animal Genetic Resources, Karnal, India, for providing necessary facilities to carry out this work. Technical support received from Mr. Naresh Kumar is gratefully acknowledged.

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Authors and Affiliations

  1. National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India

    Praveen Kumar Dubey, Shubham Goyal, Shailendra Kumar Mishra, Reena Arora, Manishi Mukesh, Saket Kumar Niranjan, Periasamy Kathiravan & Ranjit Singh Kataria

  2. Immune regulation, WPI-IFREC, Osaka University, Osaka, Japan

    Praveen Kumar Dubey

  3. Omics Application Technology Group, RIKEN Center for Life Science Technologies, Yokohama, Japan

    Shubham Goyal

  4. Animal Production and Health Section, International Atomic Energy Agency, Vienna, Austria

    Periasamy Kathiravan

  5. Animal Biotechnology Division, National Bureau of Animal Genetic Resources, P.O. Box 129, GT Road By-Pass, Karnal, 132001, Haryana, India

    Ranjit Singh Kataria

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  1. Praveen Kumar Dubey
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  2. Shubham Goyal
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  3. Shailendra Kumar Mishra
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  4. Reena Arora
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  5. Manishi Mukesh
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  6. Saket Kumar Niranjan
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  7. Periasamy Kathiravan
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  8. Ranjit Singh Kataria
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Correspondence to Ranjit Singh Kataria.

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Dubey, P.K., Goyal, S., Mishra, S.K. et al. Identification of polymorphism in fatty acid binding protein 3 (FABP3) gene and its association with milk fat traits in riverine buffalo (Bubalus bubalis). Trop Anim Health Prod 48, 849–853 (2016). https://doi.org/10.1007/s11250-016-1017-6

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  • DOI: https://doi.org/10.1007/s11250-016-1017-6

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