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Alleviation of chronic heat stress in broilers by dietary supplementation of betaine and turmeric rhizome powder: dynamics of performance, leukocyte profile, humoral immunity, and antioxidant status

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Abstract

Heat stress (HS), one of the most serious climate problems of tropical and subtropical countries, negatively affects the production performance of broilers. Keeping this in view, the current study was aimed at elucidating the effects of supplementing betaine (Bet) and dried turmeric rhizome powder (TRP), either singly or in combination, on growth performance, leukocyte profile, humoral immunity, and antioxidant status in broilers kept under chronic HS. A total of 625 one-day-old Ross male chicks were randomly assigned to five treatment groups (5 replicates of 25 birds per replicate pen). From day 1, the birds were either kept at the thermoneutral zone (TN) or exposed to HS (33 ± 1 °C) to the conclusion of study, day 42. The treatment groups were as follows: thermoneutral control (TN-CON), HS-CON, HS-Bet, HS-TRP, and HS-BT (fed Bet and TRP). The results showed that decreases in body weight gain, feed intake, and increases in feed-to-gain ratio and mortality induced by HS were partially restored by dietary supplementation of Bet and TRP. The heterophil/lymphocyte ratio, total, and IgG antibody titers against sheep red blood cell for secondary responses in the HS-TRP and HS-BT groups were also similar to those of the broilers in the TN-CON group but better (P < 0.05) than for HS-CON group. An increase (P < 0.05) in serum concentration of malondialdehyde induced by HS was significantly decreased by dietary supplementations. The serum glutathione peroxidase and superoxide dismutase activities were also higher (P < 0.05) in the supplemented groups compared to both TN and HS-CON groups. In conclusion, dietary supplementation of either Bet or TRP alone or in combination can partially ameliorate some of the detrimental effects of HS in broilers. Results also suggest that TRP might be better than Bet for improving stress tolerance and immune response in heat-stressed broilers.

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References

  • Abou-Elkhair, R., Ahmed, H.A. and Selim, S., 2014. Effects of black pepper (Piper Nigrum), turmeric powder (Curcuma Longa) and coriander seeds (Coriandrum Sativum) and their combinations as feed additives on growth performance, carcass traits, some blood parameters and humoral immune response of broiler chickens, Asian-Australasian Journal of Animal Science, 27, 847–854

    Article  CAS  Google Scholar 

  • Akbarian, A., Golian, A., Kermanshahi, H., De Smet, S. and Michiels, J., 2015. Antioxidant enzyme activities, plasma hormone levels and serum metabolites of finishing broiler chickens reared under high ambient temperature and fed lemon and orange peel extracts and Curcuma xanthorrhiza essential oil, Journal of Animal Physiology and Animal Nutrition, 99, 150–162

    Article  PubMed  CAS  Google Scholar 

  • Alirezaei, M., Gheisari, H.R., Ranjbar, V.R. and Hajibemani, A., 2012a. Betaine: a promising antioxidant agent for enhancement of broiler meat quality, British Poultry Science, 53, 699-707

    Article  PubMed  CAS  Google Scholar 

  • Alirezaei, M., Jelodar, G. and Ghayemi, Z., 2012b. Antioxidant defense of betaine against oxidative stress induced by ethanol in the rat testes, International Journal of Peptide Research and Therapeutics, 18, 239-247

    Article  CAS  Google Scholar 

  • Attia, Y.A., Hassan, R.A. and Qota, E.M.A., 2009. Recovery from adverse effects of heat stress on slow-growing chicks in the tropics 1: Effect of ascorbic acid and different levels of betaine, Tropical Animal Health and Production, 41, 807–818

    Article  PubMed  CAS  Google Scholar 

  • Bartlett, J.R. and Smith, M.O., 2003. Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress. Poultry Science, 82, 1580–1588

    Article  PubMed  CAS  Google Scholar 

  • Daneshyar, M., Kermanshahi, H. and Golian, A., 2012. The effects of turmeric supplementation on antioxidant status, blood gas indices and mortality in broiler chickens with T3-induced ascites, British Poultry Science, 53, 379-38

    Article  PubMed  CAS  Google Scholar 

  • Deminice, R., da Silva, R.P., Lamarre, S.G., Kelly, K.B., Jacobs, R.L., Brosnan, M.E. and Brosnan, J.T., 2015. Betaine supplementation prevents fatty liver induced by a high‑fat diet: effects on one-carbon metabolism, Amino Acids, 47, 839–846

  • Faghani, M., Rafiee, A., Namjoo, A. R. and Rahimian, Y., 2014. Performance, cholesterol profile and intestinal microbial population in broilers fed turmeric extract, Research Opinions in Animal and Veterinary Sciences, 4, 500-503

    Google Scholar 

  • Feng, Y., Yang, X.J., Wang, Y.B., Li, W.L., Liu, Y., Yin, R.Q. and Yao, J.H., 2012. Effects of immune stress on performance parameters, intestinal enzyme activity and mRNA expression of intestinal transporters in broiler chickens, Asian-Australasian Journal of Animal Science, 25, 701–707

    Article  CAS  Google Scholar 

  • Günal, M., 2013. The effects of early-age thermal manipulation and daily short-term fasting on performance and body temperatures in broiler exposed to heat stress, Journal of Animal Physiology and Animal Nutrition, 97, 854–860

    Google Scholar 

  • Habibi, R., Sadeghi, G.H. and Karimi, A., 2014. Effect of different concentrations of ginger root powder and its essential oil on growth performance, serum metabolites and antioxidant status in broiler chicks under heat stress, British Poultry Science, 55, 228-237

    Article  PubMed  CAS  Google Scholar 

  • Haldar, S., Ghosh, T.K., Toshiwati and Bedford, M.R., 2011. Effects of yeast (Saccharomyces cerevisiae) and yeast protein concentrate on production performance of broiler chickens exposed to heat stress and challenged with Salmonella enteritidis, Animal Feed Science and Technology, 168, 61–71

  • He, S., Zhao, S., Dai, S., Liu, D. and Bokhari, S.G., 2015. Effects of dietary betaine on growth performance, fat deposition and serum lipids in broilers subjected to chronic heat stress, Animal Science Journal, doi: 10.1111/asj.12372

    Google Scholar 

  • Huang, C., Jiao, H., Song, Z., Zhao, J., Wang, X. and Lin, H., 2015. Heat stress impairs mitochondria functions and induces oxidative injury in broiler chickens, Journal of Animal Science, 93, 2144-2153

    Article  PubMed  CAS  Google Scholar 

  • Khan, R.U., Naz, S., Nikousefat, Z., Selvaggi M., Laudadio, V. and Tufarelli V., 2012. Effect of ascorbic acid in heat-stressed poultry, World's Poultry Science Journal, 68, 477–490

    Article  Google Scholar 

  • Kim, D.K., Lillehoj, H.S., Lee, S.H., Jang, S.I., Lillehoj, E.P. and Bravo, D., 2013. Dietary Curcuma longa enhances resistance against Eimeria maxima and Eimeria tenella infections in chickens, Poultry Science, 92, 2635-2643

    Article  PubMed  CAS  Google Scholar 

  • Lee, S.H., Lillehoj, H.S., Jang, S.I., Lee, K.W., Bravo, D. and Lillehoj, E.P., 2011. Effects of dietary supplementation with phytonutrients on vaccine-stimulated immunity against infection with Eimeria tenella, Veterinary Parasitology, 181, 97-105

    Article  PubMed  CAS  Google Scholar 

  • Ling, J., Wei, B., Lv, G., Ji. H. and Li, S., 2012. Anti-hyperlipidaemic and antioxidant effects of turmeric oil in hyperlipidaemic rats, Food Chemistry, 130, 229–235

  • Lucas, A.M. and Jamroz, C., 1961. Atlas of Avian Hematology. Agriculture Monograph 25. USDA, Washington, DC

  • Mead, R., Curnow, R.N. and Hasted, A.M., 1993. Statistical methods in agriculture and experimental biology, 2nd ed. Chapman and Hall, London, UK

    Book  Google Scholar 

  • Nouzarian, R., Tabeidian, S.A., Toghyani, M., Ghalamkari, G. and Toghyani, M., 2011. Effect of turmeric powder on performance, carcass traits, humoral immune responses, and serum metabolites in broiler chickens, Journal of Animal and Feed Sciences, 20, 389–400

    Google Scholar 

  • Pamoka, S., Aengwanich, W. and Komutrin, T., 2009. Adaptation to oxidative stress and impact of chronic oxidative stress on immunity in heat-stressed broilers, Journal of Thermal Biology, 34, 353–357

    Article  Google Scholar 

  • Renaudeau, D., Collin, A., Yahav, S., de Basilio, V., Gourdine, J.L. and Collier, R.J., 2012. Adaptation to hot climate and strategies to alleviate heat stress in livestock production, Animal, 6, 707–728

    Article  PubMed  CAS  Google Scholar 

  • Rukayadi, Y. and Hwang, J.K., 2013. In vitro activity of xanthorrhizol isolated from the rhizome of javanese turmeric (Curcuma xanthorrhiza Roxb.) against candida albicans biofilms, Phytotherapy Research, 27, 1061–1066

    Article  PubMed  CAS  Google Scholar 

  • Sakomura, N.K., Barbosa, N.A.A., da Silva, E.P., Longo, F.A., Kawauchi, I.M. and Fernandes, J.B.K., 2013. Effect of betaine supplementation in diets for broiler chickens on thermoneutral environment, Revista Brasileira de Ciências Agrárias, 8, 336–341

    Article  Google Scholar 

  • Salami, S.A., Majoka, M.A., Saha, S., Garber, A. and Gabarrou, J.F. 2015. Efficacy of dietary antioxidants on broiler oxidative stress, performance and meat quality: science and market, Avian Biology Research, 8, 65-78

    Google Scholar 

  • Schmidt, K.L., Furlonger, A.A., Lapierre, J.M., MacDougall-Shackleton, E.A. and MacDougall-Shackleton, S.A., 2012. Regulation of the HPA axis is related to song complexity and measures of phenotypic quality in song sparrows, Hormones and Behavior, 61, 652–659

    Article  PubMed  CAS  Google Scholar 

  • Seevers, P.M. and Daly, J.M., 1970. Studies on the stem rust resistance controlled at the Srb locus. II Peroxidase activities, Phytopathology, 60, 1642–1647

  • Sohail, M.U., Ijaz, A., Yousaf, M.S., Ashraf, K., Zaneb, H., Aleem, M. and Rehman, H., 2010. Alleviation of cyclic heat stress in broilers by dietary supplementation of mannan-oligosaccharide and Lactobacillus-based probiotic: Dynamics of cortisol, thyroid hormones, cholesterol, C-reactive protein, and humoral immunity, Poultry Science, 89, 1934–1938

    Article  PubMed  CAS  Google Scholar 

  • Sohail, M.U., Rahman, Z.U., Ijaz, A., Yousaf, M.S., Ashraf, K., Yaqub, T., Zaneb, H., Anwar, H. and Rehman, H., 2011. Single or combined effects of mannan-oligosaccharides and probiotic supplements on the total oxidants, total antioxidants, enzymatic antioxidants, liver enzymes, and serum trace minerals in cyclic heat-stressed broilers, Poultry Science, 90, 2573–2577

    Article  PubMed  CAS  Google Scholar 

  • Song, J., Jiao, L.F., Xiao, K., Luan, Z.S., Hu, C.H., Shi, B. and Zhan, X.A., 2013. Cello-oligosaccharide ameliorates heat stress-induced impairment of intestinal microflora, morphology and barrier integrity in broilers, Animal Feed Science and Technology, 185, 175-181

    Article  CAS  Google Scholar 

  • Tan, G.Y., Yang, L., Fu, Y.Q., Feng, J.H. and Zhang, M.H., 2010. Effects of different acute high ambient temperatures on function of hepatic mitochondrial respiration, antioxidative enzymes, and oxidative injury in broiler chickens, Poultry Science, 89, 115–122

    Article  PubMed  CAS  Google Scholar 

  • Thring, S.A., Hili, P. and Naughton, D.P., 2011. Antioxidant and potential anti-inflammatory activity of extracts and formulations of white tea, rose, and with hazel on primary human dermal fibroblast cells, Journal of Inflammation, 8, 27

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Trujillo, J., Chirino, Y.I., Molina-Jijón, E., Andérica-Romero, A., Tapia E. and Chaverrí, J.P., 2013. Renoprotective effect of the antioxidant curcumin: Recent findings, Redox Biology, 1, 448-456

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Wegmann, T. and Smithies, O., 1966. A simple hemagglutination system requiring small amounts of red cells and antibodies, Transfusion, 6, 67–75

    Article  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge the financial support (Grant no. 51375930826003) for this study that was provided by the Islamic Azad University, Khoy Branch (Khoy, Iran).

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Authors and Affiliations

  1. Department of Animal Science, Islamic Azad University, Khoy Branch, Khoy, Iran

    Hossein Akhavan-Salamat

  2. Department of Animal Science, Faculty of Agriculture and Natural Resources, Arak University, 38156-8-8349, Arak, Iran

    Hossein Ali Ghasemi

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  1. Hossein Akhavan-Salamat
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Correspondence to Hossein Ali Ghasemi.

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Akhavan-Salamat, H., Ghasemi, H.A. Alleviation of chronic heat stress in broilers by dietary supplementation of betaine and turmeric rhizome powder: dynamics of performance, leukocyte profile, humoral immunity, and antioxidant status. Trop Anim Health Prod 48, 181–188 (2016). https://doi.org/10.1007/s11250-015-0941-1

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