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Procoagulant microparticles are associated with arterial disease in patients with systemic lupus erythematosus

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Abstract

Microparticles (MPs) have been associated with inflammatory and thrombotic disease. High levels of MPs have been identified in patients with systemic lupus erythematosus (SLE) and associated with cardiovascular disease. We analyzed the procoagulant activity of MPs and its correlation with arteriosclerosis and arterial thrombosis in SLE patients. Eighty-seven patients with SLE were included: 22 (25.3%) with associated antiphospholipid syndrome (APS), 32 (36.8%) without antiphospholipid antibodies (aPL) and 33 (37.9%) with aPL but without APS. Subclinical arteriosclerosis, defined as the presence and number of plaques, was evaluated by ultrasonography of carotid arteries. Thrombotic events were confirmed by objective methods. The procoagulant activity of MPs was determined by a functional assay with annexin V. Subclinical arteriosclerosis was found in 19 (21.8%) patients. Thirteen episodes of arterial thrombosis and eight of venous thrombosis were recorded. The procoagulant activity of MPs was greater in patients with arterial thrombosis (17.28 ± 8.29 nM vs 12.96 ± 7.90 nM, p < 0.05). In patients without arterial thrombosis, greater procoagulant activity of MPs was identified in patients with multiple (≥ 2) carotid plaques (17.26 ± 10.63 nM vs 12.78 ± 7.15 nM, p = 0.04). In the multivariate analysis, the procoagulant activity of MPs was independently associated with multiple (≥ 2) carotid plaques and arterial thrombosis [OR = 1.094 (95%CI 1.010–1.185), p = 0.027 and OR = 1.101 (95%CI 1.025–1.182), p = 0.008; respectively]. In conclusion, the procoagulant activity of MPs is associated with arteriosclerosis burden and arterial thrombosis in patients with SLE.

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Abbreviations

MPs:

Microparticles

NS:

No significant

nM PS eq:

Nanomolar phosphatidylserine equivalent

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Funding

This study was supported in part by Grant Nos. FIS 11/00977 and 05/0204 from the “Instituto de Salud Carlos III (ISCIII)”.

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Authors and Affiliations

  1. Department of Autoimmune Diseases, Hospital Clinic, Barcelona, Spain

    Miguel Angel Plasín-Rodríguez, Patricia Patricio, Ricard Cervera & Gerard Espinosa

  2. Department of Hemostasis and Hemotherapy, Hospital Clinic, Villarroel 170, 08036, Barcelona, Spain

    Joan Monteagudo, Joan Carles Reverter & Dolors Tàssies

  3. Department of Radiology, Centre de Diagnòstic per la Imatge, Hospital Clinic, Barcelona, Spain

    Angeles García-Criado

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  1. Miguel Angel Plasín-Rodríguez
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  2. Patricia Patricio
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Correspondence to Dolors Tàssies.

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The study was approved by the Clinical Research Ethics Committee of the Hospital Clinic de Barcelona (linked to the University of Barcelona), it was performed according to the principles of the Declaration of Helsinki.

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Plasín-Rodríguez, M.A., Patricio, P., Monteagudo, J. et al. Procoagulant microparticles are associated with arterial disease in patients with systemic lupus erythematosus. J Thromb Thrombolysis 52, 30–41 (2021). https://doi.org/10.1007/s11239-020-02295-1

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