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The systematic position of Wallinia spp. and Margotrema spp. (Digenea), parasites of Middle-American and Neotropical freshwater fishes, based on the 28S ribosomal RNA gene

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Abstract

The systematic relationships of aspinose allocreadiid-like digeneans from Middle-American and Neotropical freshwater fishes, such as Wallinia Pearse, 1920, Margotrema Lamothe-Argumedo, 1970, Magnivitellinum Kloss, 1966 and other related genera, remain enigmatic. Wallinia (2 spp.) and Margotrema (2 spp.) have been placed in the subfamily Walliniinae, within the Macroderoididae. Recent descriptions of species in these genera have followed this classification but have raised questions about their affinities with macroderoidids. Partial sequences of the 28S rRNA gene of Wallinia chavarriae Choudhury, Hartvigsen & Brooks, 2002 and Margotrema bravoae Lamothe Argumedo, 1970, as representatives of their genera, were used in a phylogenetic analysis along with the available sequences of macroderoidids and allocreadiids. Results from Maximum Parsimony, Maximum Likelihood and Bayesian Analyses were identical in placing both genera into one clade along with allocreadiids rather than the macroderoidids. Margotrema was the sister group of Crepidostomum Braun, 1900, and Wallinia was the sister group of both Margotrema and Crepidostomum combined. Bootstrap and Bremer values, as well as posterior probabilities derived from Bayesian analysis strongly supported these relationships in three analyses.

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Acknowledgements

We thank Luis García-Prieto, Instituto de Biología, UNAM, Mexico, for providing literature. We are very grateful to the following persons for their help in the field: Omar Domínguez-Domínguez, Berenit Mendoza-Garfías, Lorena Garrido-Olvera and Elizabeth Martínez-Salazar, Instituto de Biología, UNAM. Laura Márquez provided technical assistance with the sequencer and Virginia León-Régagnon access to the Molecular Systematics Laboratory. Thanks are also due to Ulises Razo-Mendivil for help with sequencing methods. This study was supported by a grant from PAPIIT-UNAM (IN220605) and CONACyT No. 47233 to G.P.P. L.; and R.R.V. thanks CONACyT and DGEP for a PhD scholarship. H.M.M. thanks PAEP 201302 for providing funds for his PhD studies. A.C. acknowledges the Faculty Development Summer Grants (2002 and 2003) from St Norbert College.

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Authors and Affiliations

  1. Laboratorio de Helmintología, Instituto de Biología, UNAM, Ap. Postal 70–153, México, D.F., C.P 04510, Mexico

    G. Pérez-Ponce de León, R. Rosas-Valdez & H. Mejía-Madrid

  2. Division of Natural Sciences, St. Norbert College, 100 Grant Street, DePere, WI, 54115, USA

    A. Choudhury

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  1. G. Pérez-Ponce de León
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  2. A. Choudhury
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  3. R. Rosas-Valdez
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  4. H. Mejía-Madrid
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Correspondence to G. Pérez-Ponce de León.

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Pérez-Ponce de León, G., Choudhury, A., Rosas-Valdez, R. et al. The systematic position of Wallinia spp. and Margotrema spp. (Digenea), parasites of Middle-American and Neotropical freshwater fishes, based on the 28S ribosomal RNA gene. Syst Parasitol 68, 49–55 (2007). https://doi.org/10.1007/s11230-006-9081-3

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