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Effect of Purple Sweet Potato Anthocyanins on β-Amyloid-Mediated PC-12 Cells Death by Inhibition of Oxidative Stress

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Abstract

Amyloid-beta peptide (Aβ) is known to induce the redox imbalance, mitochondrial dysfunction and caspase activation, resulting in neuronal cell death. Treatment with antioxidants provided a new therapeutic strategy for Alzheimer’s disease (AD) patients. Here we investigate the effects of purple sweet potato anthocyanins (PSPA), the known strong free radical scavengers, on Aβ toxicity in PC12 cells. The results showed that pretreatment of PC12 cells with PSPA reduced Aβ-induced toxicity, intracellular reactive oxygen species (ROS) generation and lipid peroxidation dose-dependently. In parallel, cell apoptosis triggered by Aβ characterized with the DNA fragmentation and caspase-3 activity were also inhibited by PSPA. The concentration of intracellular Ca2+ and membrane potential loss associated with cell apoptosis were attenuated by PSPA. These results suggested that PSPA could protect the PC-12 cell from Aβ-induced injury through the inhibition of oxidative damage, intracellular calcium influx, mitochondria dysfunction and ultimately inhibition of cell apoptosis. The present study indicates that PSPA may be a promising approach for the treatment of AD and other oxidative-stress-related neurodegenerative diseases.

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Abbreviations

PSPA:

Purple sweet potato anthocyanins

Aβ:

β-Amyloid

AD:

Alzheimer’s disease

PC12:

Pheochromocytoma cells

DMSO:

Dimethylsulfoxide

DCF-DA:

Dichlorofluorescin diacetate

MDA:

Malonyl dialdehyde

MTT:

3(4,5-dimethylthiazol-2yl)2,5-diphenyl-2H-tetrazolium bromide

OD:

Optical density

PBS:

Phosphate-buffered saline

ROS:

Reactive oxygen species

LPO:

Lipid peroxidation

RFU:

Relative fluorescence unit

[Ca2+]i:

Changes in intracellular free calcium levels

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Acknowledgments

This work was supported by a grant from the Science and Technology Bureau of Qingdao (No. 03-2-JZ-03) and the Science Foundation of Shandong Provincial Educational Department, China (Grant No. J04E17).

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Authors and Affiliations

  1. Department of Pathophysiology, Medical College, Qingdao University, Qingdao, Shandong, 266021, China

    Junli Ye & Chunling Yan

  2. Department of Psychological Consultation, No. 7 People’s Hospital of Qingdao, Qingdao, Shandong, 266034, China

    Xiangjun Meng

  3. Department of Pharmacology, Medical College, Qingdao University, 422 Room, Boya Building, 308 Ningxia Road, Qingdao, 266071, China

    Chunbo Wang

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  1. Junli Ye
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  2. Xiangjun Meng
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Corresponding author

Correspondence to Chunbo Wang.

Additional information

Junli Ye and Xiangjun Meng contributed equally to this work.

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Ye, J., Meng, X., Yan, C. et al. Effect of Purple Sweet Potato Anthocyanins on β-Amyloid-Mediated PC-12 Cells Death by Inhibition of Oxidative Stress. Neurochem Res 35, 357–365 (2010). https://doi.org/10.1007/s11064-009-0063-0

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