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The association of SNPs in Hsp90β gene 5′ flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds

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Abstract

Thermo stress induces heat shock proteins (HSPs) expression and HSP90 family is one of them that has been reported to involve in cellular protection against heat stress. But whether there is any association of genetic variation in the Hsp90β gene in chicken with thermo tolerance is still unknown. Direct sequencing was used to detect possible SNPs in Hsp90β gene 5′ flanking region in 3 chicken breeds (n = 663). Six mutations, among which 2 SNPs were chosen and genotypes were analyzed with PCR–RFLP method, were found in Hsp90β gene in these 3 chicken breeds. Association analysis indicated that SNP of C.−141G>A in the 5′ flanking region of the Hsp90β gene in chicken had some effect on thermo tolerance traits, which may be a potential molecular marker of thermo tolerance, and the genotype GG was the thermo tolerance genotype. Hsp90β gene mRNA expression in different tissues detected by quantitative real-time PCR assay were demonstrated to be tissue dependent, implying that different tissues have distinct sensibilities to thermo stress. Besides, it was shown time specific and varieties differences. The expression of Hsp90β mRNA in Lingshan chickens in some tissues including heart, liver, brain and spleen were significantly higher or lower than that of White Recessive Rock (WRR). In this study, we presume that these mutations could be used in marker assisted selection for anti-heat stress chickens in our breeding program, and WRR were vulnerable to tropical thermo stress whereas Lingshan chickens were well adapted.

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Acknowledgments

This study was supported by grants from the National Natural Scientific Foundation of China (30972093).

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Authors and Affiliations

  1. College of Animal Science, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Zhuo-Yu Chen, Jian-Kang Gan, Xiong Xiao, Li-Yan Jiang, Xi-Quan Zhang & Qing-Bin Luo

  2. Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Zhuo-Yu Chen, Jian-Kang Gan, Xiong Xiao, Li-Yan Jiang, Xi-Quan Zhang & Qing-Bin Luo

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  1. Zhuo-Yu Chen
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Correspondence to Qing-Bin Luo.

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Chen, ZY., Gan, JK., Xiao, X. et al. The association of SNPs in Hsp90β gene 5′ flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds. Mol Biol Rep 40, 5295–5306 (2013). https://doi.org/10.1007/s11033-013-2630-3

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