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Diversity array technology (DArT) 56K analysis, confirmed by SNP markers, distinguishes one сrested wheatgrass Agropyron species from two others found in Kazakhstan

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Abstract

Molecular genetic polymorphism in three species and four subspecies of crested wheatgrass, Agropyron, was studied using 56K diversity array technology (DArT), and the results confirmed with four selected SNP Amplifluor markers. In total, 82 accessions from three species—A. desertorum, A. fragile, and two subspecies of A. cristatum (ssp. cristatum and ssp. pectinatum)—were collected from various regions of Kazakhstan or ordered from Genebank in Russia, for morphological taxonomy and molecular phylogenetic analyses. In the DArT clone analysis, two Agropyron species with narrow linear spikes, A. fragile and A. desertorum, were found to be genetically similar and fell within a single clade (A). Both species share similar eco-geographical origins. All samples of A. cristatum including the two subspecies, ssp. pectinatum and ssp. cristatum, which have short broad spikes, were interspersed within two other clades, B and C, more genetically distanced from the other species. Four SNP Amplifluor markers developed for genetic fragments on different chromosomes confirmed the distinction between the studied species. These results, derived from multiple molecular markers, suggest that the morphological taxonomy of these Agropyron species should be re-considered carefully in the future.

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Acknowledgements

We wish to thank the staff and students of S. Seifullin Kazakh AgroTechnical University, Astana (Kazakhstan) for their support in this research. We also thank Carly Schramm for critical comments in the manuscript.

Funding

Research project no. 3733/GF4 has been supported by Ministry of Education and Science (Kazakhstan).

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Authors and Affiliations

  1. Kostanai Research Institute of Agriculture, Kostanay, Kazakhstan

    Talgat Absattar

  2. National Agricultural Research Education Centre, Astana, Kazakhstan

    Aiman Absattarova

  3. Scientific and Production Centre of Grain Farming, Shortandy, Kazakhstan

    Nadezhda Fillipova

  4. Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Astana, Kazakhstan

    Aimur Otemissova

  5. School of Biological Sciences, Flinders University, Bedford Park, SA, Australia

    Yuri Shavrukov

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  1. Talgat Absattar
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  2. Aiman Absattarova
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  3. Nadezhda Fillipova
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  4. Aimur Otemissova
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  5. Yuri Shavrukov
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Correspondence to Yuri Shavrukov.

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Absattar, T., Absattarova, A., Fillipova, N. et al. Diversity array technology (DArT) 56K analysis, confirmed by SNP markers, distinguishes one сrested wheatgrass Agropyron species from two others found in Kazakhstan. Mol Breeding 38, 37 (2018). https://doi.org/10.1007/s11032-018-0792-3

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