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Effects of palmitic acid on lipid metabolism homeostasis and apoptosis in goose primary hepatocytes

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Abstract

Studies have shown that not only does palmitic acid promote triglyceride (TG) accumulation, but it also affects cell viability in in vitro steatosis models. However, to what degree these effects are mediated by steatosis in goose primary hepatocytes is unknown. In this study, the effects of palmitic acid on the lipid metabolism homeostasis pathway and on apoptosis were determined. The authors measured the mRNA levels of genes involved in TG synthesis, lipid deposition, fatty acid oxidation and the assembly and secretion of VLDL–TG in goose primary hepatocytes. The results indicated that palmitic acid can significantly reduce the activity of goose hepatocytes, and that palmitic acid had a significant effect on TG accumulation; however, with increasing palmitic acid concentrations, the extracellular TG and extracellular VLDL concentration gradually decreased. With increasing palmitic acid concentrations, the gene expression levels of DGAT1, DGAT2, PPARα, CPT-1, FoxO1 and MTTP (which regulate hepatic TG synthesis, fatty acid oxidation and the assembly and secretion of VLDL–TGs) first increased and then decreased; the change in PLIN gene expression was palmitic acid dose-dependent, similar to the regulatory mode of intracellular TG accumulation. In conclusion, this study clearly shows that palmitic acid can promote TG accumulation and induce apoptosis in goose primary hepatocytes, and this effect may be related to the lipid metabolism pathway.

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Abbreviations

VLDL:

Very low-density lipoprotein

TG:

Triglyceride

DGAT:

Acyl CoA:diacylglycerol acyltransferase

MTTP:

Microsomal triglyceride transfer protein

FoxO1:

Forkhead box O1

PPARs:

Peroxisome proliferator-activated receptors

PLIN:

Perilipin

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Acknowledgment

The study was supported by the earmarked fund for Modern Agro-industry Technology Research System, no: NYCYTX-45-05.

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Authors and Affiliations

  1. Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, 625014, Sichuan, People’s Republic of China

    Zhixiong Pan, Jiwen Wang, Hui Tang, Liang Li, Jia Lv, Lu Xia, Chunchun Han, Feng Xu, Hua He, Hengyong Xu & Bo Kang

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  1. Zhixiong Pan
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  2. Jiwen Wang
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Correspondence to Jiwen Wang.

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Pan, Z., Wang, J., Tang, H. et al. Effects of palmitic acid on lipid metabolism homeostasis and apoptosis in goose primary hepatocytes. Mol Cell Biochem 350, 39–46 (2011). https://doi.org/10.1007/s11010-010-0680-6

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  • DOI: https://doi.org/10.1007/s11010-010-0680-6

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