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Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto_3016 from protein domain family PF04237 (DUF419) adopt a “double wing” DNA binding motif

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Journal of Structural and Functional Genomics

Abstract

The protein Pspto_3016 is a 117-residue member of the protein domain family PF04237 (DUF419), which is to date a functionally uncharacterized family of proteins. In this report, we describe the structure of Pspto_3016 from Pseudomonas syringae solved by both solution NMR and X-ray crystallography at 2.5 Å resolution. In both cases, the structure of Pspto_3016 adopts a “double wing” α/β sandwich fold similar to that of protein YjbR from Escherichia coli and to the C-terminal DNA binding domain of the MotA transcription factor (MotCF) from T4 bacteriophage, along with other uncharacterized proteins. Pspto_3016 was selected by the Protein Structure Initiative of the National Institutes of Health and the Northeast Structural Genomics Consortium (NESG ID PsR293).

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Acknowledgments

The authors thank John A. Schwanof and Randy Abramowitz for their support at the X4 beam line, National Synchrotron Light Source, Brookhaven National Lab. This work was supported by grants from the National Institute of General Medical Sciences Protein Structure Initiative (PSI) of the National Institutes of Health, PSI-2 (P50 GM 074958) and PSI:Biology (U54-GM094597). NMR data collection was conducted at the Ohio Center of Excellence in Biomedicine in Structural Biology and Metabonomics at Miami University.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Miami University, Oxford, OH, 45056, USA

    Erik A. Feldmann, Theresa A. Ramelot & Michael A. Kennedy

  2. Northeast Structural Genomics Consortium, Piscataway, NJ, USA

    Erik A. Feldmann, Jayaraman Seetharaman, Theresa A. Ramelot, Scott Lew, Keith Hamilton, Colleen Ciccosanti, Rong Xiao, Thomas B. Acton, John K. Everett, Liang Tong, Gaetano T. Montelione & Michael A. Kennedy

  3. Department of Biological Sciences, Columbia University, New York, NY, 10027, USA

    Jayaraman Seetharaman, Scott Lew & Liang Tong

  4. Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA

    Li Zhao, Keith Hamilton, Colleen Ciccosanti, Rong Xiao, Thomas B. Acton, John K. Everett & Gaetano T. Montelione

  5. Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ, 08854, USA

    Gaetano T. Montelione

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  1. Erik A. Feldmann
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Correspondence to Michael A. Kennedy.

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Feldmann, E.A., Seetharaman, J., Ramelot, T.A. et al. Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto_3016 from protein domain family PF04237 (DUF419) adopt a “double wing” DNA binding motif. J Struct Funct Genomics 13, 155–162 (2012). https://doi.org/10.1007/s10969-012-9140-8

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  • DOI: https://doi.org/10.1007/s10969-012-9140-8

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