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Determination of Angiotensin-(1–7) with HPLC/Fluorescence-Detection

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Abstract

Angiotensin-(1–7) is an important active component in the renin-angiotensin-system. Due to its cardio protective effects it is now under investigation in combination with antioxidants as a reperfusion solution. The combination showed impressive effects on isolated hearts of male Wistar rats after induced ischemia. In this work a high performance liquid chromatography method with fluorescence detection was developed for the first time for in-process measurements as well as for stability tests of the peptide in the novel antioxidant-containing Karal® solution. For fluorescence detection of angiotensin-(1–7) fluorescamine as derivatization dye was applied. Under optimized conditions the method showed linearity over the range of 50 to 5000 ng/mL with R2 of 0.9988 and an overall precision better than 5.0 %. LOD and LOQ were determined to be in the femtomol range on column. It was found that stability of angiotensin-(1–7) could be significantly improved in the antioxidant containing preparation compared to aqueous solutions.

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Authors and Affiliations

  1. Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Schubertstraße 1, 8010, Graz, Austria

    Miriam Russ, Susanne Hauser, Reinhold Wintersteiger & Astrid Ortner

  2. Institute of Physiological Chemistry, Medical University Graz, Harrachgasse 21, 8010, Graz, Austria

    Joachim Greilberger

  3. Division of Transplantation Surgery, Medical University Graz, Auenbruggerplatz 29, 8036, Graz, Austria

    Michaela Andrä

Authors
  1. Miriam Russ
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  2. Susanne Hauser
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  3. Reinhold Wintersteiger
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  4. Joachim Greilberger
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  5. Michaela Andrä
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  6. Astrid Ortner
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Correspondence to Astrid Ortner.

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Russ, M., Hauser, S., Wintersteiger, R. et al. Determination of Angiotensin-(1–7) with HPLC/Fluorescence-Detection. J Fluoresc 26, 81–86 (2016). https://doi.org/10.1007/s10895-015-1686-y

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  • DOI: https://doi.org/10.1007/s10895-015-1686-y

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