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Influence of 3D porous galactose containing PVA/gelatin hydrogel scaffolds on three-dimensional spheroidal morphology of hepatocytes

  • Tissue Engineering Constructs and Cell Substrates
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Abstract

Three-dimensional liver scaffolds are temporary framework that mimics native ECM architecture and positively influence hepatocyte lodging, proliferation with retention of metabolic activities. The aim of the current study is to develop galactose containing physical cross-linked polyvinyl alcohol/gelatin (P/G 8:2 and 9:1) hydrogel scaffolds via freeze/thaw technique. The 8:2 and 9:1 P/G hydrogels exhibited comparable pore size and porosity (P > 0.05). The tensile strength of the fabricated 8:2 and 9:1 P/G hydrogel scaffolds were found to be in accordance with native human liver. Pore interconnectivity of both the P/G hydrogel scaffolds was confirmed by scanning electron micrographs and liquid displacement method. Further galactose containing hydrogel promoted cell–cell and cell-hydrogel interaction, aiding cellular aggregation leading to spheroids formation compared to void P/G hydrogel by 7 days. Hence, galactose containing P/G hydrogel could be more promising substrate as it showed significantly higher cell proliferation and albumin secretion for 21 days when compared to non-galactose P/G hydrogels (P < 0.05).

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Acknowledgments

The author wish to acknowledge the Indian Council for Medical Research (35/22/2010-BMS), Nano Mission (SR/S5/NM-07/2006 & SR/NM/PG-16/2007) and FIST program (SR/FST/LSI-327/2007) of the Department of Science & Technology, Govt. of India for funding. Prof. T. R. Rajagopalan R & D Cell of SASTRA University is also acknowledged.

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  1. Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical & Biotechnology, SASTRA University, Thanjavur, 613 401, Tamil Nadu, India

    Kirthanashri S. Vasanthan, Anuradha Subramaniam, Uma Maheswari Krishnan & Swaminathan Sethuraman

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  1. Kirthanashri S. Vasanthan
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  2. Anuradha Subramaniam
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Correspondence to Swaminathan Sethuraman.

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Vasanthan, K.S., Subramaniam, A., Krishnan, U.M. et al. Influence of 3D porous galactose containing PVA/gelatin hydrogel scaffolds on three-dimensional spheroidal morphology of hepatocytes. J Mater Sci: Mater Med 26, 20 (2015). https://doi.org/10.1007/s10856-014-5345-7

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  • DOI: https://doi.org/10.1007/s10856-014-5345-7

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