Abstract
To allow to discriminate rapidly the strains of Porphyra tenera var. tamatsuensis, cultivars of which grow more vigorously than strains of P. tenera var. tenera, strains of both varieties were examined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis using mitochondrial DNA related to the ATP synthase F0 subunit 6 (ATP6) gene. The lengths of all sequences in this region of three strains of each variety were 670 bp and had just a single nucleotide substitution. Digestion with the restriction enzyme of TaaI yielded three visible bands which appeared in the three strains of P. tenera var. tamatsuensis, whereas two bands appeared in the three strains of P. tenera var. tenera. We therefore conclude that PCR-RFLP analysis is a valuable tool for discrimination of P. tenera var. tamatsuensis among the stock of P. tenera strains used for mariculture.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abe M, Kobayashi M, Tamaki M, Fujiyoshi E, Kikuchi N (2008) Discrimination of Porphyra tenera Kjellman var. tamatsuensis Miura (Bangiales, Rhodophyta) based on partial sequence of mitochondrial DNA related to ATP6 gene—a preliminary study. Suisanzoshoku 56:497–503
Kikuchi N, Yoshida T, Yoshinaga K (2002) Habitat of threatened species of Porphyra (Bangiales, Rhodophyta) in Japan. Ecosophia 9:112–117 (In Japanese)
Miura A (1984) A new variety and a new form of Porphyra (Bangiales, Rhodophyta) from Japan: Porphyra tenera Kjellman var. tamatsuensis Miura, var. nov. and P. yezoensis Ueda form. narawaensis Miura, form. nov. J Tokyo Univ Fish 71:1–37
Niwa K, Furuita H, Yamamoto T, Kobiyama A (2008) Identification and characterization of a green-type mutant of Porphyra tenera Kjellman var. tamatsuensis Miura (Bangiales, Rhodophyta). Aquaculture 274:126–131
Niwa K, Kikuchi N, Aruga Y (2005a) Morphological and molecular analysis of the endangered species Porphyra tenera (Bangiales, Rhodophyta). J Phycol 41:294–304
Niwa K, Kobiyama A, Aruga Y (2005b) Confirmation of cultivated Porphyra tenera (Bangiales, Rhodophyta) by polymerase chain reaction restriction fragment length polymorphism analyses of the plastid and nuclear DNA. Phycol Res 53:296–302
Ofusa T (2001) Illustrated current state and future of nori industry. Seizando-Shoten, Tokyo, In Japanese
Yoshida T, Kikuchi N, Yoshinaga K (1999) Wild population of Porphyra tenera Kjellman. Jpn J Phycol 47:119–122 (In Japanese with English abstract)
Acknowledgments
The authors wish to express our thanks to Dr. Kyosuke Niwa, Fisheries Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries for valuable advice in this study. We also thank Kabashima Suisan, Ariakekai Laboratory, Fukuoka Fisheries and Marine Technology Research Center, Kumamoto Prefectural Fisheries Research Center, Yamaguchi Prefectural Fisheries Research Center, Inland Sea Research Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency and Professor Miyuki Maegawa, Graduate School of Bioresources, Mie University for collecting samples.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Abe, M., Kobayashi, M., Tamaki, M. et al. Rapid discrimination of Porphyra tenera Kjellman var. tamatsuensis Miura by PCR-RFLP. J Appl Phycol 22, 405–408 (2010). https://doi.org/10.1007/s10811-009-9472-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10811-009-9472-3