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The Galectin-1 level in serum as a novel marker for stress

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Abstract

Galectin-1(Gal-1), a carbohydrate-binding protein with an affinity for β-galactoside, is widely expressed in various normal and pathological tissues and it also plays an important role in regulating immune cell homeostasis and tumorigenesis. This study investigated the effects of restraint stress on serum Gal-1 by Western blot analyses and enzyme-linked immunosorbent assays. The Gal-1 levels of the restraint-stress group were significantly higher than those of the control group. However, this increase by stress was not obvious in adolescent rats. The pattern of these changes was similar to that of corticosterone. Furthermore, this Gal-1 increase in the serum was prevented by pre-treatment with a neurotoxin 6-hydroxydopamine (6-OHDA), which destroys the noradrenergic nerve terminals. However, a bilateral adrenalectomy (ADX) had no effect on the Gal-1 increase. These results suggest that Gal-1 is a candidate stress marker protein and that the stress-induced increase of Gal-1 in serum is regulated by the sympathetic nervous system under stress conditions.

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References

  1. Eskandari, F., Sternberg, E.M.: Neural-immune interactions in health and disease. Ann. N. Y. Acad. Sci. 966, 20–27 (2002)

    Article  PubMed  CAS  Google Scholar 

  2. Arakawa, H., Kodama, H., Matsuoka, N., Yamaguchi, I.: Stress increases plasma enzyme activity in rats: differential effects of adrenergic and cholinergic blockades. J. Pharmacol. Exp. Ther. 280(3), 1296–1303 (1997)

    PubMed  CAS  Google Scholar 

  3. Pan, B., Castro-Lopes, J.M., Coimbra, A.: Chemical sensory deafferentation abolishes hypothalamic pituitary activation induced by noxious stimulation or electroacupuncture but only decreases that caused by immobilization stress. A c-fos study. Neuroscience 78(4), 1059–1068 (1997)

    Article  PubMed  CAS  Google Scholar 

  4. de Paula Brotto, M.A.: Temporal effects of stress by immobilization and sensitivity of the isolated rat pacemaker to isoproterenol: roles of corticosterone, neuronal uptake, and beta-adrenergic homogeneity. J. Pharmacol. Exp. Ther. 306(3), 1152–1158 (2003)

    Article  PubMed  CAS  Google Scholar 

  5. Ng, V., Koh, D., Mok, B.Y., Chia, S.E., Lim, L.P.: Salivary biomarkers associated with academic assessment stress among dental undergraduates. J. Dent. Educ. 67(10), 1091–1094 (2003)

    PubMed  Google Scholar 

  6. Konishi, N., Otaka, M., Odashima, M., Jin, M., Wada, I., Komatsu, K., Sato, T., Kato, S., Matsuhashi, T., Watanabe, S.: Systemic stress increases serum leptin level. J. Gastroenterol. Hepatol. 21(7), 1099–1102 (2006)

    Article  PubMed  CAS  Google Scholar 

  7. Romeo, R.D., Bellani, R., Karatsoreos, I.N., Chhua, N., Vernov, M., Conrad, C.D., McEwen, B.S.: Stress history and pubertal development interact to shape hypothalamic-pituitary-adrenal axis plasticity. Endocrinology 147(4), 1664–1674 (2006)

    Article  PubMed  CAS  Google Scholar 

  8. Lucas, L.R., Wang, C.J., McCall, T.J., McEwen, B.S.: Effects of immobilization stress on neurochemical markers in the motivational system of the male rat. Brain Res. 1155, 108–115 (2007)

    Article  PubMed  CAS  Google Scholar 

  9. Ohashi, T., Matsui, T., Chujo, M., Nagao, M.: Restraint stress up-regulates expression of zinc transporter Zip14 mRNA in mouse liver. Cytotechnology 57(2), 181–185 (2008)

    Article  PubMed  CAS  Google Scholar 

  10. Torner, L., Karg, S., Blume, A., Kandasamy, M., Kuhn, H.G., Winkler, J., Aigner, L., Neumann, I.D.: Prolactin prevents chronic stress-induced decrease of adult hippocampal neurogenesis and promotes neuronal fate. J. Neurosci. 29(6), 1826–1833 (2009)

    Article  PubMed  CAS  Google Scholar 

  11. Mizoguchi, K., Yuzurihara, M., Ishige, A., Sasaki, H., Chui, D.H., Tabira, T.: Chronic stress differentially regulates glucocorticoid negative feedback response in rats. Psychoneuroendocrinology 26(5), 443–459 (2001)

    Article  PubMed  CAS  Google Scholar 

  12. Camby, I., Le Mercier, M., Lefranc, F., Kiss, R.: Galectin-1: a small protein with major functions. Glycobiology 16(11), 137R–157R (2006)

    Article  PubMed  CAS  Google Scholar 

  13. Scott, K., Weinberg, C.: Galectin-1: a bifunctional regulator of cellular proliferation. Glycoconj. J. 19(7–9), 467–477 (2004)

    PubMed  Google Scholar 

  14. Saussez, S., Lorfevre, F., Lequeux, T., Laurent, G., Chantrain, G., Vertongen, F., Toubeau, G., Decaestecker, C., Kiss, R.: The determination of the levels of circulating galectin-1 and-3 in HNSCC patients could be used to monitor tumor progression and/or responses to therapy. Oral. Oncol. 44(1), 86–93 (2008)

    Article  PubMed  CAS  Google Scholar 

  15. Kopcow, H.D., Rosetti, F., Leung, Y., Allan, D.S., Kutok, J.L., Strominger, J.L.: T cell apoptosis at the maternal-fetal interface in early human pregnancy, involvement of galectin-1. Proc. Natl. Acad. Sci. U. S. A. 105(47), 18472–18477 (2008)

    Article  PubMed  CAS  Google Scholar 

  16. Ishibashi, S., Kuroiwa, T., Sakaguchi, M., Sun, L., Kadoya, T., Okano, H., Mizusawa, H.: Galectin-1 regulates neurogenesis in the subventricular zone and promotes functional recovery after stroke. Exp. Neurol. 207(2), 302–313 (2007)

    Article  PubMed  CAS  Google Scholar 

  17. Almkvist, J., Karlsson, A.: Galectins as inflammatory mediators. Glycoconj. J. 19(7–9), 575–581 (2004)

    PubMed  Google Scholar 

  18. Horie, H., Inagaki, Y., Sohma, Y., Nozawa, R., Okawa, K., Hasegawa, M., Muramatsu, N., Kawano, H., Horie, M., Koyama, H., Sakai, I., Takeshita, K., Kowada, Y., Takano, M., Kadoya, T.: Galectin-1 regulates initial axonal growth in peripheral nerves after axotomy. J. Neurosci. 19(22), 9964–9974 (1999)

    PubMed  CAS  Google Scholar 

  19. Choufani, G., Nagy, N., Saussez, S., Marchant, H., Bisschop, P., Burchert, M., Danguy, A., Louryan, S., Salmon, I., Gabius, H.J., Kiss, R., Hassid, S.: The levels of expression of galectin-1, galectin-3, and the Thomsen-Friedenreich antigen and their binding sites decrease as clinical aggressiveness increases in head and neck cancers. Cancer 86(11), 2353–2363 (1999)

    Article  PubMed  CAS  Google Scholar 

  20. Mine, K., Nakagawa, T., Fujiwara, M., Ito, Y., Kataoka, Y., Watanabe, S., Ueki, S.: A new experimental model of stress ulcers employing aggressive behavior in 6-OHDA-treated rats. Physiol. Behav. 27(4), 715–721 (1981)

    Article  PubMed  CAS  Google Scholar 

  21. Nadeau, R.A., de Champlain, J., Tremblay, G.M.: Supersensitivity of the isolated rat heart after chemical sympathectomy with 6-hydroxydopamine. Can. J. Physiol. Pharmacol. 49(1), 36–44 (1971)

    Article  PubMed  CAS  Google Scholar 

  22. de Champlain, J., Nadeau, R.: 6-hydroxydopamine, 6-hydroxydopa and degeneration of adrenergic nerves. Fed. Proc. 30(3), 877–885 (1971)

    PubMed  Google Scholar 

  23. Choi, S., Kellogg, C.K.: Adolescent development influences functional responsiveness of noradrenergic projections to the hypothalamus in male rats. Brain Res. Dev. Brain Res. 94(2), 144–151 (1996)

    Article  PubMed  CAS  Google Scholar 

  24. Rabinovich, G.A., Sotomayor, C.E., Riera, C.M., Bianco, I., Correa, S.G.: Evidence of a role for galectin-1 in acute inflammation. Eur. J. Immunol. 30(5), 1331–1339 (2000)

    Article  PubMed  CAS  Google Scholar 

  25. Rabinovich, G.A., Ariel, A., Hershkoviz, R., Hirabayashi, J., Kasai, K.I., Lider, O.: Specific inhibition of T-cell adhesion to extracellular matrix and proinflammatory cytokine secretion by human recombinant galectin-1. Immunology 97(1), 100–106 (1999)

    Article  PubMed  CAS  Google Scholar 

  26. van der Leij, J., van den Berg, A., Blokzijl, T., Harms, G., van Goor, H., Zwiers, P., van Weeghel, R., Poppema, S., Visser, L.: Dimeric galectin-1 induces IL-10 production in T-lymphocytes: an important tool in the regulation of the immune response. J. Pathol. 204(5), 511–518 (2004)

    Article  PubMed  Google Scholar 

  27. Horie, H., Kadoya, T., Hikawa, N., Sango, K., Inoue, H., Takeshita, K., Asawa, R., Hiroi, T., Sato, M., Yoshioka, T., Ishikawa, Y.: Oxidized galectin-1 stimulates macrophages to promote axonal regeneration in peripheral nerves after axotomy. J. Neurosci. 24(8), 1873–1880 (2004)

    Article  PubMed  CAS  Google Scholar 

  28. Tsai, C.M., Chiu, Y.K., Hsu, T.L., Lin, I.Y., Hsieh, S.L., Lin, K.I.: Galectin-1 promotes immunoglobulin production during plasma cell differentiation. J. Immunol. 181(7), 4570–4579 (2008)

    PubMed  CAS  Google Scholar 

  29. Rabinovich, G.A., Riera, C.M., Sotomayor, C.E.: Galectin-1, an alternative signal for T cell death, is increased in activated macrophages. Braz. J. Med. Biol. Res. 32(5), 557–567 (1999)

    Article  PubMed  CAS  Google Scholar 

  30. Kristensen, D.B., Kawada, N., Imamura, K., Miyamoto, Y., Tateno, C., Seki, S., Kuroki, T., Yoshizato, K.: Proteome analysis of rat hepatic stellate cells. Hepatology 32(2), 268–377 (2000)

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was carried out at the Kanagawa Dental College, Research Center of Advanced Technology for Craniomandibular Function, and supported by a grant-in-aid for Bio-venture Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Department of Craniofacial Growth and Development Dentistry, Kanagawa Dental College, 82 Inaoka-Cho, Yokosuka, Kanagawa, 238-8580, Japan

    Mayuko Iwamoto, Chiho Taguchi, Kenichi Sasaguri & Sadao Sato

  2. Faculty of Care and Rehabilitation, Seijoh University, 2-172 Fukinodai, Tohkai, Aichi, 476-8588, Japan

    Kin-ya Kubo

  3. Research Center of Brain Research and Oral Science, Kanagawa Dental College, 82 Inaoka-Cho, Yokosuka, Kanagawa, 238-8580, Japan

    Hidenori Horie

  4. Department of Human Biology, Kanagawa Dental College, 82 Inaoka-Cho, Yokosuka, Kanagawa, 238-8580, Japan

    Toshiharu Yamamoto

  5. Department of Physiology and Neuroscience, Kanagawa Dental College, 82 Inaoka-Cho, Yokosuka, Kanagawa, 238-8580, Japan

    Minoru Onozuka

  6. Department of Biotechnology, Maebashi Institute of Technology, 460-1 Kamisadori-machi, Maebashi, Gunma, 371-0816, Japan

    Toshihiko Kadoya

Authors
  1. Mayuko Iwamoto
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Correspondence to Kenichi Sasaguri.

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Iwamoto, M., Taguchi, C., Sasaguri, K. et al. The Galectin-1 level in serum as a novel marker for stress. Glycoconj J 27, 419–425 (2010). https://doi.org/10.1007/s10719-010-9288-z

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  • DOI: https://doi.org/10.1007/s10719-010-9288-z

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